| 应用两种麦管系统对小鼠囊胚的玻璃化冷冻 |
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| 关 键 词： | 小鼠 囊胚 玻璃化冷冻 开放性麦管系统 辅助生殖 |
Vitrification of mouse blastocysts using two kinds of straw systems] |
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| Authors: | Liang Zhu Chen Lou Min-zhen Huang Hong Li Fu-qi Xing |
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| Institution: | Center of Assisted Reproduction, Nanfang Hospital, Guangzhou 510515, China. 9_9gy@163.com |
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| Abstract: | OBJECTIVE: To compare open pulled straws with conventional sorans system for the purpose of establishing an effective vitrification method for mouse blastocysts. METHODS: With open pulled straws (OPS) and conventional straws, vitrifi- cation of mouse blastocysts was performed, and the survival and hatching rates of the blastocysts were observed after thawing. RESULTS: After vitrification, the embryo survival in the OPS group (82.3%) was significantly higher than conventional straw group (68.6%, P<0.01), and the hatching rate was also higher (70.3%, P<5 CONCLUSIONS: Vitrification of mouse blastocysts using OPS results in higher survival and hatching rates as compared with conventional straw method. |
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