重组脂联素对糖尿病肾病大鼠氧化应激的影响

目的探讨脂联素在早期糖尿病肾病中可能的作用机制。方法采用高糖高脂饮食加小剂量链脲佐菌素（STZ）30mg/kg的方法建立2型糖尿病肾病大鼠模型,并以正常大鼠为对照组。将造模成功的糖尿病肾病大鼠模型再随机分为3组：糖尿病肾病组（DN组,n=6）,空载体转染组（DP组,腹腔注射脂质体＋pIRES2-EGFP,200μg/次,每周两次）和脂联素转染组（DA组,腹腔注射脂质体＋pIRES2-EGFP-gAd,200μg/次,每周两次）。10周后处死大鼠测肾组织羟自由基、丙二醛（MDA）含量,光镜观察肾脏病理改变。结果与正常对照组比较,模型组各组大鼠肾组织抑制羟自由基能力降低（P〈0.01）,丙二醛增加（P〈0.05）;与DN、DP组大鼠比较,DA组大鼠肾组织抑制羟自由基能力升高,丙二醛减少,均有明显统计学意义（P〈0.05）。结论脂联素干预糖尿病肾病大鼠,可抑制肾脏羟自由基产生,使脂质过氧化物生成减少,具有一定的抗氧化能力,对糖尿病肾病可能有一定的保护作用。

Study of recombinant adiponectin on oxidative stress in diabetic nephropathy rats

Objective To explore the role of adiponectin in early diabetic nephropathy. Methods Rat model of diabetic nephropathy was established by sucrose and fat-enriched diet and intraperitoneal administration of low-dose streptozotocin （STZ, 30 mg/kg）. The model rats were randomly divided into 3 groups： diabetic nephropathy rats without any other treatment （DN group, n=6）, diabetic nephropathy rats intraperitoneally injected with the plasmid vector pIRES2-EGFP 200μg twice a week （DP group）, and diabetic nephropathy rats intraperitoneally injected with the recombinant plasmid pIRES2-EGFP-gAd 200μg twice a week （DA group）. After the treatment for 10 weeks, rats were sacrificed, hydroxyl flee radicals and malonic dialdehyde （MDA） in renal tissue were measured, and pathological change in kidney was examined by microscopy. Results The scavenging ability of hydroxyl free radicals was decreased （P 〈 0.01） and MDA was increased （P 〈 0.05） in DN group as compared with those of normal controls. The scavenging ability of hydroxyl free radicals was increased （P 〈 0.05） and MDA was decreased in DA group as compared with those in DN and DP groups （P 〈 0.05）. Conclusion Administration of adiponectin to diabetic nephropathy rats reduces hydroxyl free radicals and MDA in the kidney. Adiponectin inhibits oxidative stress in the kidney, and is useful to prevent the kideny from diabetic nephropathy.