一对父亲与胎儿所携乙型肝炎病毒前S基因的序列分析

目的：筛选一位父亲HBsAg,HBeAg，抗HBc均阳性的慢性携带者和母亲无任何乙型肝炎病毒（HBV）标志的子宫内受染胎儿为研究对象，分析父、儿所携带HBV前S基因的基因序列，探讨HBV男性垂直传播的存在。方法：应用半套式PCR检测父亲、胎儿的PreS1/S2基因，将扩增的片段克隆到PGEM－T载体进行克隆测序，并与文献中的中国人参照株序列相比较，结果：父、儿血清中均扩增出前S基因，测序结果表明二者的基因同源性达99％以上，且均有183bp的PreS1基因缺失，而与中国人参照株序列的同源性为89％，结论：父、儿前S序列的高度一致性表明HBV极可能存在男性垂直传播。

SEQUENCING ANALYSIS OF THE PreS_1/S_2 GENE OF HEPATITIS B VIRUS BETWEEN A FATHER AND HIS SON.

Objective:To study the possibility of HBV vertical transmission from father to fetus by the analysis of HBV PreS 1/S 2 gene sequence.Method:the PreS 1/S 2 genes extracted from serums of a father whose wife had not any HBV markers and his fetus were amplified by nested polymerase chain reaction(PCR),and the positive PCR products were cloned and sequenced.The acquired sequences were compared with chinese consensus sequence reported by Guo Yabing.Results:The father and his fetus were positive.The nucleotide homology were 99% between the father and his fetus,both of them lost 183 base pair in PreS 1.Compared with consensus sequence,they were 89% homology.Conclusion:The high homology between the sequences of the father and his fetus revealed that the existence of vertical transmission from father to fetus was very possibe.