JNK抑制剂对鼠实验性牙周病后基质金属蛋白酶影响的研究

目的 研究JNK特异性抑制剂SP600125对大鼠实验性牙周病后基质金属蛋白酶的影响。方法 18只雄性SD大鼠随机分为对照组、生理盐水组(腹腔注射生理盐水)和SP600125组(腹腔注射SP600125),每组6只。每只大鼠右侧下颌第一磨牙牙颈部结扎钢丝,4周后去除钢丝,对照组在全麻下切取牙龈组织。此后生理盐水组每天腹腔注射生理盐水,SP600125组每天腹腔注射SP600125稀释液,连续7d后分别在全麻下切取牙龈组织。利用明胶酶谱法,分别检测对照组、生理盐水组和SP600125组牙龈组织中MMP-2的酶活性。结果 SP600125组牙龈组织中MMP-2酶活性较对照组和生理盐水组明显降低,差异有统计学意义。结论 JNK特异性抑制剂SP600125能够降低实验性鼠牙周病牙龈组织中的MMP-2酶活性,进一步证实JNK传导通路在牙周病的形成和发展中起到重要作用,本研究可为牙周病的治疗提供一条新的途径。?

Effects of JNK inhibitor on matrix metalloproteinas after experimental periodontitis model in rat

Objective To evaluate the effects of JNK specific inhibitor-SP600125 on the matrix metalloproteinas after experimental periodontitis model in rat.Methods Eighteen rats were divided randomly into control group,Sodium Chloride group and SP600125 group.Steel-wire was ligated around the cervical of each right first maxillary molar for four weeks.And then,SP600125 group were injected with SP600125 through abdominal cavity every day for seven days,and Sodium Chloride were injected with Sodium Chloride through abdominal cavity every day for seven days.The activity of MMP-2 was evaluated by gelatin zymography.Results The MMP-2 of SP600125 group were decreased more than those of the Sodium Chloride group and control group.Conclutions JNK inhibitor-SP600125 could reduce the MMP-2 of experimental periodontitis model in rat.It is further confirmed that JNK-mediated pathway may have played an important role in the formation and development of paradentosis.So this experiment probably provides a novel therapeutic way for periodontitis.