The VP3 gene of human group C rotavirus |
| |
Authors: | Ali Reza Samarbaf-Zadeh Paul R. Lambden Steve M. Green Yu Deng E. Owen Caul Ian N. Clarke |
| |
Affiliation: | (1) Molecular Microbiology, University Medical School, Southampton General Hospital, SO16 6YD Southampton, United Kingdom;(2) Regional Virus Laboratory, Myrtle Road, BS2 8EL Bristol, United Kingdom |
| |
Abstract: | The complete nucleotide sequence of genome segment 4 from the human group C rotavirus (Bristol strain) was determined. Comparison of the nucleotide sequences of the genome termini with the consensus 5 and 3 terminal non-coding sequences of the human group C rotavirus genome revealed characteristic 5 and 3 sequence motifs. Human group C rotavirus genome segment 4 is 2, 166bp long and encodes a single open reading frame of 2,082 nucleotides (693 amino acids) starting at nucleotide 55 and terminating at nucleotide 2,136 giving a 3 untranslated region of 30 nucleotides. Alignment with the porcine group C VP3 equivalent gene showed the human gene is one amino acid longer, and that the proteins have 84.1% amino acid sequence identity. A conserved potential nucleotide binding motif shared with the porcine VP3 sequence was identified. Analogy with the group A rotaviruses suggested that the genome segment 4 encodes the group C rotavirus guanylyltransferase. |
| |
Keywords: | Rotavirus dsRNA VP3 gene |
本文献已被 SpringerLink 等数据库收录! |
|