霍乱弧菌抑制子基因及间隔区(rstR-ig2)多态性研究

目的研究霍乱弧菌CTXφ基因组抑制子基因及间隔区（rstR-ig2）多态性。方法聚合酶链反应（PCR）及限制性片段长度多态性分析（PCR-RFLP）、型特异性引物PCR分型。结果霍乱弧菌（VC）01群古典型（CVC）5株均为class类型。VC01群埃尔托型（EVC）155株共分为4个类型：class、ET、class＋ET、class ＋newET，后2个类型为首次发现。不同地区分离的EVC均以ET类型为主，以class和class＋ET类型为辅。O139群VC82株共分为4个类型：ET、ET＋new O139、ET＋calc、ET＋calc＋newO139，后3个类型为首次发现；福建省分离的菌株以ET4-newO139类型为主，其它地区分离的均以ET类型为主。结论EVC和O139群VC的rstR-ig2基因均以ET类型为主．首次发现5种不同类型rstR-ig2基因共存的新类型。

Polymorphism of Repressor Gene and Intergenic 2 Gene(rstR-ig2)of Vibrio cholerae Strains

Objective To study the polymorphism of repressor gene and intergenic 2 gene(rstR-ig2)of Vibrio cholerae strains.Method Polymorphism of Vibrio cholerae strains were analyzed by PCR-RFLP and PCR using type specific primers.Result 5 strains of classical biotype of Vibrio cholerae(VC)O1 serogroup(CVC)were all classical type.155 strains of El Tor biotype of O1 serogroup(EVC)were divided into four types(class,ET,class ET,class new ET).The last two types were the first time found in these areas.ET type was the main type of EVC found in different areas,and class and class ET was second major type.82 strains of O139 serogroup VC were divided into four types(ET,ET new O139,ET calc,ET calc new O139).The last three types were first time found in these areas.The main type of strains isolated from Fujian province was ET newO139,while the main type of strains isolated from other districts was ET.Conclusion Amount the EVC and O139 serogroup,the rstR-ig2 genes were belong to the ET type.Five types were found for the first time.