川芎天麻伍用镇痛、镇静作用的实验研究

目的]探索川芎天麻镇痛、镇静作用的最佳配伍比例。方法]按析因设计的方法设置16个不同的配伍比例。采用热板法与醋酸扭体法来观察各配伍比例对小鼠的镇痛作用;采用旷场实验与悬尾实验来观察各配伍比例对大鼠的镇静作用。结果]川芎∶天麻为1∶3(3 g/kg)可以显著提高小鼠在热板实验中的痛阈值(P0.01);川芎∶天麻为1∶4(3 g/kg)可以显著减少小鼠腹腔注射醋酸溶液后的扭体次数(P0.01);川芎∶天麻为1∶4(2 g/kg)可以显著降低大鼠在旷场实验仪中的自主运动活性(P0.01);川芎∶天麻为0∶4(2 g/kg)可以显著缩短大鼠在悬尾实验中的不动时间(P0.01)。结论]川芎、天麻配伍可以对小鼠和大鼠产生镇痛、镇静作用,其中川芎、天麻最佳配伍比例为1∶4。

Experiment research on the analgesic effect and sedative effect based on the compatibility of Rhizoma ligusticum and Gastrodia elata in different proportions

Objective] Observe the analgesic and the sedative activity based on the compatibility of Rhizoma ligusticum and Gastrodia elata in different proportions;seek the best proportion of the compatibility of Rhizoma ligusticum and Gastrodia elata.Methods] According to the factorial design method to set up 16 different compatibility proportions.To observe the analgesic effect of different compatibility proportions on mice by hot plate test and acetic acid writhing test.With the open field test and tail suspension test to observe the sedative effects of different compatibility proportions on rats.Results] The ninth group (Rhizoma ligusticum:Gastrodia elata is 1:3,3 g/kg) can significantly increase the pain threshold in hot plate test in mice (P<0.01).The tenth group (Rhizoma ligusticum:Gastrodia elata is 1:4,3 g/kg) can significantly reduce the writhing times of mice after intraperitoneal injection of acetic acid solution (P<0.01).The tenth group (Rhizoma ligusticum:Gastrodia elata is 1:4,2 g/kg) can significantly reduce the independent movement activity in the open field test instrument in rats (P<0.01).The fifth group (Rhizoma ligusticum:Gastrodia elata is 0:4,2 g/kg) can significantly shorten the immobility time in tail suspension of rats in the experiment (P<0.01).Conclusion] The compatibility of Rhizoma ligusticum and Gastrodia elata can produce sedative and analgesic effect in mice and rats,in which the best compatibility proportion group is 1:4.