Down‐Regulation of Asparagine Synthetase Induces Cell Cycle Arrest and Inhibits Cell Proliferation of Breast Cancer |
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| Authors: | Yabing Zheng Weiliang Feng Xianghou Xia Xingfei Yu Zhiqiang Lin |
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| Affiliation: | 1. Department of Internal Oncology, Zhejiang Cancer Hospital, , Hangzhou, 310022, China;2. Department of Breast Surgery, Zhejiang Cancer Hospital, , Hangzhou, 310022 China;3. Zhejiang Cancer Research Institute, Zhejiang Cancer Hospital, , Hangzhou, 310022 China |
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| Abstract: | Asparagine synthetase (ASNS) is deemed to be a promising therapeutic target for the treatment of several cancers, but its functional role in human breast cancer is still unknown. In this study, we employed RNA interference as an efficient tool to silence endogenous ASNS expression in breast cancer cell lines. The relationship between ASNS expression and breast cancer cell growth was investigated, and the therapeutic value of ASNS in breast cancer was further evaluated. Depletion of ASNS remarkably inhibited the proliferation and colony formation capacity of breast cancer cells and arrested cell cycle in the S phase. Our findings suggest that ASNS may contribute to breast cancer tumorigenesis and could be a potential therapeutic target in human breast cancer. |
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| Keywords: | asparagine synthetase breast cancer cell proliferation RNA interference |
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