Some properties of carnation ringspot virus single- and double-stranded ribonucleic acid |
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| Authors: | J.A. Dodds J.H. Tremaine W.P. Ronald |
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| Affiliation: | 1. Botany Department, University of British Columbia, Vancouver, British Columbia Canada;2. Research Station, Agriculture Canada, 6660 Northwest Marine Drive, Vancouver, British Columbia, Canada V6T 1X2 |
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| Abstract: | From sodium dodecyl sulfate (SDS)-dissociated carnation ringspot virus (CRSV) two RNAs of different sizes were separated by density gradient centrifugation. Preparations of the smaller RNA-2 (0.5 × 106 daltons) were not infectious; preparations of the larger RNA-1 (1.5 × 106 daltons) were only moderately infectious. Mixtures of the two kinds of preparations were very infective. This enhancement of infectivity was demonstrated within and between the two RNAs from each of two strains of CRSV. The genetic information controlling irreversible virus particle aggregation and dissociation of virus particles by SDS at pH 5 was contained in RNA-1. Virus-infected tissue contained dsRNA replicative forms corresponding to RNA-1, RNA-2, and a minor ss-RNA component. |
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| Keywords: | Send reprint requests to J. H. Tremaine at Research Station Agriculture Canada. |
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