The uptake and secretion of 2-acetylaminofluorene by the rat and dog prostate

In unanesthetized rats examined 4–140 hr after the ip administration of 1.8 mg/kg of [9-¹⁴C]2-acetylaminofluorene ([¹⁴C]2-AAF), detectable amounts of radioactivity were found in the plasma and in the ventral and dorsolateral lobes of the prostate. Radioactivity was also found in the prostatic fluid collected from anesthetized rats between 25 and 29 hr after doses of 2 mg/kg. When three unanesthetized dogs with surgically prepared fistulas allowing the collection of prostatic fluid were given intraperitoneal doses of 0.16–0.25 mg/kg of [¹⁴C]2-AAF and followed for 6 hr (two dogs) or 166 hr (one dog), there was a relatively rapid rise in the amount of radioactivity in plasma followed by a slow decline and an appearance of radioactivity in the prostatic fluid. Radioactivity was also present in the prostate glands of each of two dogs examined 6 hr after treatment. Thus 2-AAF and/or metabolites were found to enter both the prostate gland and the prostatic secretion of both the rat and dog.