茶叶的化学成分

目的分离并鉴定茶叶(Camellia sinensis)中的化学成分。方法采用硅胶柱色谱、羟丙基葡聚糖凝胶(Sephadex LH-20)柱色谱和重结晶等多种分离方法对体积分数为70%的乙醇溶液回流提取物进行分离,并通过NMR和MS等多种谱学方法对分离得到的化学成分进行结构鉴定。结果分离得到8个化合物,分别鉴定为咖啡碱(caffeine,1)、可可碱(theobromine,2)、山柰酚(kaempferol,3)、(+)儿茶素((+)-catechin,4)、胡萝卜苷(daucosterol,5)、(Z)-9-十六碳烯酸((Z)-9-hexadecenoic acid,6),苯基-β-萘胺(phenyl-β-naphthylamine,7)和没食子酸(gallic acid,8)。结论其中化合物5、6和7为首次从该属植物中分离得到。

Chemical constituents from the leaves of Camellia sinensis

Objective To isolate the chemical constituents and elucidate their structures from the leaves of Camellia sinensis.Methods The compounds were isolated by chromatography on silica gel,Sephadex LH-20 column and recrystallizing methods.NMR and MS were employed for the structure identification.Results Eight compounds were obtained and elucidated as:caffeine(1),theobromine(2),kaempferol(3),(+)-catechin(4),daucosterol(5),(Z)-9-hexadecenoic acid(6),phenyl-β-naphthylamine(7),gallic acid(8),respectively.Conclusions Among these compounds,5,6 and 7 are obtained from the Camellia genus for the first time.