EcoRI polymorphism of the metastasis-suppressor gene NME1 in Mexican patients with breast cancer |
| |
Authors: | Susan Andrea Gutierrez Rubio Silvia Esperanza Flores Martinez Jose Sanchez Corona Adriana Patricia Mendizabal Ruiz Angel Emilio Suarez Rincon Ignacio Arevalo Lagunas Jose Gonzalo Vazquez Camacho Maria Cristina Moran Moguel |
| |
Institution: | (1) Medicina Molecular Division, Centro de Investigacion Biomedica de Occidente, Guadalajara, Mexico;(2) Medicina Molecular Division, Centro de Investigacion Biomedica de Occidente, Sierra Mojada #800, col Independencia, Jalisco, 44340 Guadalajara, Mexico |
| |
Abstract: | Summary Human breast cancer cells with high metastatic potential show reduced expression of the metastasis-suppressor gene NME1. There are two polymorphic sites for the restriction enzymes BglII and EcoRI, both detectable by Southern blot analysis. Although the BglII site has been analyzed for loss of heterozygosity, the biallelic EcoRI site polymorphism has not been studied in association with breast cancer, complications or metastasis. We analyzed EcoRI site allele frequencies in Mexican patients with breast cancer, using polymerase chain reaction -restriction fragment length
polymorphisms. The polymorphic allelic frequencies in the cases and reference groups were 0.4215 and 0.3375, respectively;
this difference was not statistically significant (χ2=0.8687, p=0.3512). Thus, EcoR1 polymorphic site was not associated with breast cancer in this series, but could be analyzed in association with metastases
and might be informative in the evaluation of loss of heterozygosity in women with breast cancer. |
| |
Keywords: | breast cancer EcoR1 site polymorphisms metastasis-suppressor NME1 |
本文献已被 PubMed SpringerLink 等数据库收录! |
|