银杏叶提取物对大鼠局灶性脑缺血后血管新生的影响

目的探讨银杏叶提取物是否可以促进大鼠局灶性脑缺血后梗死周围区的血管新生，寻求促使脑缺血后血管新生、开发脑储备能力的有潜力的中医药疗法。方法采用线栓法建立大鼠大脑中动脉闭塞（MCAO）模型，术后24h采用银杏叶注射液以10mg／（kg体重·d）的剂量连续腹腔注射治疗6d，于缺血后第7d、14天分别观察银杏叶组和对照组大鼠脑梗死体积、脑梗死灶周围血管密度（层粘连蛋白，Laminin的表达）和神经功能缺失评分的变化。结果与对照组比较，大鼠局灶性脑缺血后第7天和14天银杏叶组的梗死体积明显减小，梗死周围血管密度较对照组明显增加，并显示出更好的神经功能评分（P〈0．05）。结论银杏叶提取物可促进局灶性脑缺血后大鼠脑梗死周围区的血管新生，从而开发脑储备能力，这可能是银杏叶提取物改善脑缺血后神经功能恢复的机制之一。

Angiogenesis effect of ginbgo biloba extract on focal cerebral ischemia in rats

Objective To investigate whether treatment with ginkgo biloba extract enhance angiogenesis after focal cerebral ischemia in rats. Methods Wistar rats were subjected to middle cerebral artery occlusion by intraluminal filament method. Ginkgo biloba extract （10 mg/kg） were given intraperitoneally daily for 6 consecutive days starting 24 h after ischemia. Size of infarction, vascular density in the peri-infarction region and score of neurologic function were examined on 7 d and 14 d after ischemia. Results Compared with control group at 7 d and 14 d after MCAO, infarction size of Ginkgo biloba extraxt group was sighificantly reduced. Meanwhile, vascular density in the peri-infarction region and score of neurologic function were obviously improved in the ginkgo biloba extraxt group （P 〈 0.05）. Conclusion Ginkgo biloba extract may improve angiogenesis after focal ischemia, which may be one of the neuroprotection mechanism of ginkgo biloba extract.