大黄酸及大黄素对人肾小管上皮细胞增殖及TGF—β1启动子活性的调控作用

目的：观察大黄酸和大黄素对人肾小管上皮细胞（HK-2）增殖及对人转化生长因子-β1（TGF-β1）基因启动子活性的作用。方法：应用MTT法观察不同浓度大黄酸和大黄素（1μg/ml、10μg/ml、50μg/ml和100μg/ml）对人肾小管上皮细胞增殖的作用；将人TGF-β1基因启动子片段与氯霉素乙酰基转移酶（CAT）报告基因组成重组体phTGF2．14，采用脂质体法转染至人肾小管上皮细胞中，分别加入不同浓度的大黄酸和大黄素（1μg/ml、10μg/ml和50μg/ml）进行干预，用ELISA方法检测报告基因CAT的活性。结果：大黄酸和大黄素各浓度组对人肾小管上皮细胞均具有明显的抑制HK-2细胞增殖的作用（P〈0．01），且呈剂量依从性；大黄酸和大黄素浓度为lμg/ml和10μg/ml时对重组体phTGF2．14的表达活性无影响，当增大其浓度为50μg/ml时对重组体phTGF2．14的表达活性有抑制作用，其抑制率分别为38．0％和36．0％，与对照组比较均有显著差异（P〈0．01）。结论：大黄酸和大黄素能够抑制人肾小管上皮细胞增殖，当浓度为50μg/ml时对人TGF-β1基因启动子活性具有一定的抑制作用。

Regulatory Effect of Rhein and Emodin on Proliferation of Human Renal Tubular Epithelial Cell Line and Activity of TGF-[~ gene Promotor

Objective ： To observe the effect of rhein and emodin on the proliferation of human renal tubular epithelial cell line （HK-2） and the activities of TGF-β1 gene promoter. Methods： Effects of rhein and emodin on proliferation of HK-2 were analyzed by MTT; different lengths of human TGF-β1 gene and CAT as reporter gene were constructed and were transferred into HK-2 RMC by transfection reagent. The effects of rhein and emodin （1 μg/ml, 10 μg/ml, 50 μg/ml and 100 μg/ml） on the plasmid were determined by CAT-ELISA. Results： Rhein and emodin of different concentrations had regulatory effect on the proliferation of HK-2 （P 〈0.01 ） , and with dose compliance; rhein and emodin at 1 μg/ml and 10 μg/ml didn＇t inhibit the activities of phTGF 2.14. Rhein and emodin at 50 μg/ml markedly inhibited the activities of ph TGF 2.14, and the inhibition ratio was 38.0% and 36.0%, was different from the control group （P 〈 0.01 ）. Conclusion： Rhein and emodin have regulatory effect on the proliferation of HK-2. Rhein and emodin at 50 μg/ml have regulatory effect on the activity of TGF-β1 gene promoter.