低氧培养对RL95-2细胞线粒体损伤及凋亡的影响

目的 探讨低氧培养对于人子宫内膜上皮腺癌细胞株（RL95-2）线粒体损伤及细胞凋亡的影响.方法 用含有不同浓度（0、10、30、62.5、125 μmol/L）氯化钴的培养液培养RL95-2细胞株.采用CCK-8法测定细胞增殖活性;JC-1荧光染色,流式细胞仪分析细胞线粒体膜电位变化;Annexin V/PI双染流式细胞仪检测细胞凋亡.结果 随着氯化钴浓度升高或作用时间延长（62.5 μmol/L作用下）, RL95-2细胞增殖活性逐渐下降,线粒体膜电位去极化增加,细胞凋亡率升高.结论 缺氧造成子宫内膜上皮细胞增殖活性下降,线粒体损伤,凋亡率升高.

Effects of hypoxia culture on damage and apoptosis of RL95-2 cell line

Objective To investigate effects of hypoxia culture on mitochondria damage and apoptosis of human endometrial epithelial carcinoma cell line (RL95-2). Methods RL95-2 cells were cultured with medium containing different concentrations of CoCl2 (0, 10, 30, 62.5, and 125 μmol/L). Cell proliferation activity, mitochondria membrane potential, and apoptosis rate were determined via CCK-8 kit, JC-1 fluorescence staining with flow cytometry, and Annex V/PI. Results With CoCl2 concentration rising or extension of action time (62.5 μmol/L), the proliferation activity of RL95-2 cells was weak, while the depolarization of mitochondria membrane and the apoptosis rate were increased. Conclusion The condition of hypoxia induces decrease of proliferation activity, mitochondria damage, increase of apoptosis of endometrial epithelial cells.