OSAHS患儿血清Th1／Th2细胞因子检测及临床意义

目的 探讨阻塞性睡眠呼吸暂停低通气综合征（OSAHS）患儿血清Th1/Th2免疫平衡相关细胞因子的检测及临床意义.方法 收集61例OSAHS患儿内镜下腺样体阻塞程度数据,并测定患儿血清中Th1细胞因子-肿瘤坏死因子β（TNF-β）和干扰素-γ（IFN-γ）,Th2细胞因子IL-10、IL-13和IL-12的水平.同时收集26名正常儿童样本为对照组,进行相同血清细胞因子检测.结果 OSAHS患儿血清IFN-γ的水平要明显低于正常对照组儿童,差异有统计学意义（P〈0.01）;但OSAHS 患儿其他血清Th1/Th2免疫平衡相关细胞因子（TNF-β、IL-10、IL-13和IL-12）与对照组之间差异无统计学意义（P〉0.05）.OSAHS患儿IFN-γ水平与其腺样体肥大阻塞后鼻孔程度之间并无相关性.结论 Th1和Th2免疫反应间存在制衡机制.儿童OSAHS病例中,血清IFN-γ的水平下降表明,由此导致Th1细胞介导的保护性细胞免疫反应下降,Th1/Th2失衡,从而使儿童腺样体扁桃体过度肥大而发病.

Detection of serum Th1/Th2 cytokines and clinical significance in children with obstructive sleep apnea-hypopnea syndrome

Objective To detect serum Helper T cell subsets (Th1/Th2) immune balance related cytokines in children with obstructive sleep apnea hypopnea syndrome (OSAHS) and explore their clinical significance. Methods Obstructive level data of 61 OSAHS children undergone endoscopic adenoidectomy were collected. The serum Th1 cytokines (TNF-β and IFN-γ), Th2 cytokines (IL-10, IL-13, and IL-12) levels were measured. The samples of 26 normal children were also collected as control group for the same serum cytokines detection. Results Compared with control group children, serum IFN-γ levels in OSAHS children were significantly lower (P<0.01). But there were no significant differences of other serum Th1/Th2 immune balance related cytokines (TNF-β, IL-10, IL-13, and IL-12) between OSAHS children and control group children (P>0.05). In OSAHS children, IFN-γ levels was not correlated with the degree of choanal adenoidal hypertrophy obstruction. Conclusion There is balance mechanism between Th1/Th2 immune response. In OSAHS children, decrease of serum IFN-γ level induced decline of Th1 cell-mediated protective immune response, which caused Th1/Th2 imbalance to result in adenoid tonsil hypertrophy.