| 检出山梨醇阳性肠出血性大肠杆菌O157:H7一例及特征分析 |
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| 引用本文： | 范放,司徒翠华,黄李华,谢昭聪. 检出山梨醇阳性肠出血性大肠杆菌O157:H7一例及特征分析[J]. 医学动物防制, 2006, 22(2): 88-90 |
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| 作者姓名： | 范放 司徒翠华 黄李华 谢昭聪 |
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| 作者单位： | 深圳出入境检验检疫局,518067 |
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| 摘 要： | 目的:肠出血性大肠杆菌O157:H7(entro-hemorrhaguc E.Coli O157:H7 or EHEC O157:H7)是具有强致病力的条件致病菌,该菌可通过污染的食物、水、以及直接接触感染的人或动物而感染。加强对该菌的检验,对预防与控制由该菌所致的感染流行十分重要。方法:本文参照中华人民共和国出入境检验检疫行业标准SN/T 0973-2000方法进行检验。结果:从进口冻猪肚中检出一例EHEC O157:H7,并用PCR方法检测VERO毒素呈阳性。结论:该菌株的生化特征与相关文献报道的EHEC O157:H7生化特征不同的是山梨醇阳性,棉籽糖阴性,检验过程中应慎重鉴别。
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| 关 键 词： | 肠出血性大肠杆菌O157:H7(EHEC O157:H7) 山梨醇阳性 VERO毒素 |
DETECTION OF ENTRO-HEMORRHAGUC E. COLIO157: H7 WITH SORBITOL-POSITIVE AND ANALYSIS |
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| FAN Fang,SITU Cui-hua,HUANG Li-hua,XIE Zhao-cong. DETECTION OF ENTRO-HEMORRHAGUC E. COLIO157: H7 WITH SORBITOL-POSITIVE AND ANALYSIS[J]. Chinese Journal of Pest Control, 2006, 22(2): 88-90 |
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| Authors: | FAN Fang SITU Cui-hua HUANG Li-hua XIE Zhao-cong |
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| Affiliation: | Shenzhen Entry-exit Inspection and Quarantine Bureau ,Shenzhen 518067,China |
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| Abstract: | Odjective: Entro-hemorrhaguc E.Coli O157:H7 can cause severe life-threatening illness and has a high infecyive.It is transmitted through contaminated food or drinking-water,as well as by infected person or animal.It is important to enhance detection for O157:H7.Methods: The standard SN/T 0973-2000,Method for determination of entero-hemorrhagic E.coliO157:H7 in meats and meat products for import and export was used to test the sample.Results: Detected one strain of EHEC O157:H7 from entry freeze pig stamach.Detection of VERO toxin was positive by PCR method.Conclusion: Biochemical characteristics of the strain is difference between the repoets of literatures.For example sorbitol-positive and raffinose-negative.In the process of detection must carefulness. |
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| Keywords: | Entro-hemorrhaguc E. Coli O157-H7 Sorbitol-positive VERO toxin |
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