金属硫蛋白基因多态性与2型糖尿病的关系

目的:探讨金属硫蛋白（metallothionein,MT）基因多态性与中国汉族人群中2型糖尿病（T2DM）发病之间的关系。 方法:采用PCR技术和限制性片段长度多态性(RFLP)的方法检测41名糖尿病患者和53名正常对照者的金属硫蛋白基因家族中的MT4基因（rs666636）201G→A突变,统计各组对象的突变频率。 结果:MT4基因（rs666636）201G→A突变型等位基因(A)频率在糖尿病组和正常对照组中差异有显著性(P<0.05,OR=2.335);AA, GA和GG三种基因型频率分布差异也有显著性 (P<0.05);不同基因型的相对风险分析， GA基因型携带者患2型糖尿病的风险是GG基因型的3.462倍(OR=3.162，95% OR CI=1.389~8.629)。 结论:MT4突变型等位基因与2型糖尿病的发生有显著的关联性，基因突变使2型糖尿病的发病风险增加。

Study on proliferation of hematopoietic cell in patients with myelodysplastic syndrome

ObjectiveTo study the proliferation of hematopoietic cell in patients with myelodysplastic syndrome (MDS). MethodsLiquid single layer culture and semisolid culture in vitro were used and flow cytometry was used to detect the cell cycle, apoptosis and the expressions of PCNA, P53,and BCL 2 Results①The Clony Forming Unit Granulocyte Macroplage(CFU GM) and the Erythroid Clony Forming Unit(CFU E) of myelodysplasyic syndromes (MDS/RA) decreased significantly ( P <0 001); while CFU GM,CFU E of MDS/RAEB could not be detectable; ②The percentage of G1 phase increased and the percentage of S, G 2/M phase decreased significantly ( P <0 01), compared with control group; ③The increase of apoptotic bodies of MDS/RA was much more markedly ( P <0 01), while the changes of those in MDS/RAEB could not be found significantly; the expression of PCNA of MDS increased in both MDS/RA and MDS/RAEB ( P <0 01), especially in MDS/RAEB group;④the protein expression of P53 in MDS increased, while BCL 2 decreased significantly in MDS, compared with normal control ( P <0 01). ConclusionThere are severe abnormalities in hematopoietic cell of patients with MDS, higher proliferation and excessive apoptosis occur in bone marrow cells and the changes of p53,bcl 2 gene suggest their possible participation in the regulation of apoptosis.