HPLC法测定乌藤镇痛胶囊中的青藤碱含量

目的建立高效液相色谱法测定乌藤镇痛胶囊中青藤碱含量的方法。方法采用色谱柱：Shim-packCLC—ODS—C18柱（150mm×4．6mm，5μm）；流动相为甲醇-磷酸盐缓冲液（5mmol／L磷酸氢二钠溶液，以5mmol／L磷酸二氢钠调节pH值至8．0，再以1％三乙胺溶液调节pH至9．0）（55：45）；流速为1．0ml／min，柱温为25℃，检测波长为262nm。结果青藤碱在0．92～4．60μg之间线性关系较好，相关系数r=0．9996。加样回收率为99．21％（RSD为0．61％）。结论本方法灵敏、准确、快速，可用于乌藤镇痛胶囊中青藤碱含量测定及质量控制。

Determination of sinomenine in Wuten Zhentong capsules by HPLC

Objective To establish an HPLC method for determining the content of sinomenine in Wuten Zhentong capsule. Methods The sample was analyzed on a Shim-pack CLC-ODS-C18 （ 150 mm × 4.6 mm,5 μm） column using methanol-phosphate buffer（5 mmol/L sodium hydrogen phosphate solution, adjust pH to 8.0 using 5 mmol/L sodium hypophosphite solution and then adjust pH to 9.0 using 1% triethylamine solution） （55 ： 45） as mobile phase at 25 ℃. The detection wavelength was 26：2 nm and flow rate 1. 0 ml/min. Results Good linearity was shown within the range of 0.92 - 4.60 ~g, the correlation coefficient （r） was 0.999 6, the average recovery rate was 99.21% with RSD of 0.61% （ n = 6）. Conclusion The results demonstrated that the analytical methods used for the content determination were reproducible, feasible, convenient and easily performable, which can be adopted as the standard methods for the quality control of the Wuten Zhentong capsule.