淋球菌对阿奇霉素耐药性与mtrR/mtrC基因变异的关联分析

目的探讨mtr系统若干基因变异与淋球菌对阿奇霉素耐药性的相关性。方法对临床分离的淋球菌阿奇霉素耐药性代表株mtrR基因序列及mtrR启动子区域回文结构序列的突变进行分析,使用荧光定量PCR技术对结构基因mtrC的表达水平进行测定。结果所有中等以上耐药菌株均发生mtrR基因H105Y的变异,伴随mtrR启动子区域回文结构的缺失突变;敏感株有一株出现G45D的突变,mtrR启动子区域回文结构未检测出突变;中等以上耐药菌株与敏感菌株相比mtrC基因表达有显著差异(P<0.05)。结论mtrR基因的H105Y变异和回文序列碱基缺失与mtrC表达有负向相关关系,与淋球菌阿奇霉素耐药性显著相关。

The Analysis on the Relationship between mtrR/mtrC Mutations and Azithromyc in-resistance of Neisseria gonorrhoeae

Objective Exploring some mutations within mtr Systen with the Azithromycin-resistance of Neisseria gonorrhoeae.Methods MtrR gene was amplified by PCR reactions,then sequenced.Real-time PCR was used to measure the expression level of mtrC gene.Results All of Azithromycin-intermediate and resistant strains except from those sensitive trains,had been detected an H105Y mutation within mtrR gene,and a base "A" deletion within its promoter region(P<0.05).The expression levef of mtrC was different among three groups(sensitive,intermediate and reisistant,P<0.01).Conclusion H105Y mutation and the base-A deletion in mtrR negatively correlated with the expression of mtrC geness.Those mutations correlated with the resistance to Azithromycin.