紫外线照射对储存血中淋巴细胞功能的影响

目的 :研究紫外线照射对储存血中淋巴细胞功能的影响 .方法 :在血标本经紫外线照射前后及储存 72h后测定淋巴细胞亚群及活性、细胞因子 .结果 :紫外线照射储存血可抑制淋巴细胞的活性 .照射后随储存时间的延长 ,血液中IL 2水平与对照组相比明显降低 (P <0 .0 5 ) ;经紫外线照射后淋巴细胞转化率较对照组明显降低 (P <0 .0 1 ) ;对照组与照射组血液在放置 72h后CD3+ ,CD4 + ,CD8+ 细胞亚群均有明显下降 ,但无显著差异 .结论 :紫外线照射储存血可抑制淋巴细胞的活性 ,对淋巴细胞的增殖能力有明显抑制作用 ;可减少细胞因子的产生和积累

Effects of ultraviolet irradiation on function of lymphocyte in banked blood

AIM: To explore the effects of ultraviolet irradiation on the function of lymphocytes in banked blood. METHODS: Changes of lymphocyte subgroups, lymphocyte activity, lymphocyte transformation, interleukin 2 and its receptor were tested before and after ultraviolet irradiation, and after storage for 72 h separately. RESULTS: Ultraviolet irradiation inhibited the lymphocyte activity. As compared with that of the control group, the content of interleukin 2 decreased significantly with prolonged storage time( P <0.05). Ultraviolet irradiation markedly inhibited the lymphocytes transformation( P <0.01). CD3 +, CD4 + and CD8 + lymphocyte subgroups in banked blood decreased as compared with those of prestorage in the two groups,but there was no significant difference between the two groups. CONCLUSION: Ultraviolet irradiation can inhibit lymphocytes activity and lymphocytes transformation and reduce the accumulation of cytokine in banked blood.