HBV X蛋白对HepG2细胞凋亡的影响

目的:初步探讨HBV X蛋白表达对转染X基因HepG2细胞的影响。方法:磷酸钙沉淀法转染真核表达质粒pCDNA3.1(+)/x到人肝癌细胞株HepG2;72 h后,Western-blot检测X蛋白表达;流式细胞仪检测细胞周期和凋亡率;MTT法测定细胞活力。结果:磷酸钙沉淀法能有效的导入X基因且Western-blot能检测到X蛋白表达;MTT法、流式细胞术结果显示X蛋白表达组细胞活力受抑制(P<0.01),凋亡率增加(P<0.01)。结论:HBV感染及其相关慢性肝病中,X蛋白的表达可能与肝细胞凋亡,炎症活性相关。

The Effects of the Expression of HBV X Gene on Apoptosis in HepG2 Cell

Objective To elucidate the effect of the expression of HBV X gene on hepatocyte apoptosis.Methods The eukaryon expression vector of x gene(pCDNA3.1-x) was introduced into human Hepatocarcinoma cell strain HepG2 with calcium phosphate precipitates transfection.MTT colorimetric assay and flow cytometric analysis of DNA histograms were used to measure the proliferation and cell cycle distribution and Cell Apoptosis of HepG2 cells.Western-blot confirmed the expression of recombine X protein.Results The expression of HBV X protein exerted the inhibitory actions on HepG2 cells(P<0.01).The increasing apoptotic rate of HepG2x was Significant compared with the controls(P<(0.01).)Conclusion HBV X gene and X protein can promote the apoptosis in HepG2 cell.