| 髓系抑制性细胞在小鼠肝癌免疫逃逸中的作用 |
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| 引用本文： | 徐晓琴,赵素莲,卫琴.髓系抑制性细胞在小鼠肝癌免疫逃逸中的作用[J].中国免疫学杂志,2010,26(6). |
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| 作者姓名： | 徐晓琴 赵素莲 卫琴 |
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| 作者单位： | 山西医科大学微生物与免疫教研室,太原,030001 |
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| 摘 要： | 目的:研究荷瘤小鼠脾脏中髓系抑制性细胞(Myeloid-derived suppressor cells,MDSCs)比例的变化及在组织中的分布,探讨其在肝癌免疫逃逸中的作用。方法:采用肝癌细胞原位移植法建立小鼠原位肝癌模型,流式细胞仪检测正常小鼠和荷瘤小鼠7、14、21天脾脏中MDSCs的比例,免疫组织化学染色对其进行定位分析。结果:荷瘤小鼠脾脏中MDSCs的比例比正常小鼠显著增高(P0.05),而且随着荷瘤时间的延长,MDSCs的比例逐渐增加。正常小鼠脾脏中少量的MDSCs散在分布于红髓区,荷瘤后大量MDSCs主要聚积在边缘区及白髓区的动脉周围淋巴鞘。结论:小鼠脾脏中MDSCs的比例与肿瘤进展相关,且主要分布在脾脏胸腺依赖区。
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| 关 键 词： | 髓系抑制性细胞 免疫逃逸 荷瘤 肝癌 |
Immune escape mediated by myeloid-derived suppressor cells in hepatocarcinoma mice |
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| XU Xiao-Qin,ZHAO Su-Lian,WEI Qin.Immune escape mediated by myeloid-derived suppressor cells in hepatocarcinoma mice[J].Chinese Journal of Immunology,2010,26(6). |
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| Authors: | XU Xiao-Qin ZHAO Su-Lian WEI Qin |
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| Institution: | XU Xiao-Qin,ZHAO Su-Lian,WEI Qin.Department of Microbiology , Immunology of Shanxi Medical University,Taiyuan 030001,China |
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| Abstract: | Objective:The changes and distribution of myeloid-derived suppressor cells (MDSCs) in spleen of tumor-bearing mice was studied,and its function in tumor escape was investigated.Methods:Orthotopic transplantation tumor model of hepatocarcinoma in mice was established.At the 7th,14th,21st day after tumor implantation,the proportion of MDSCs in spleen was tested by flow cytometry.The localization of MDSCs and semi-quantitative analysis was conducted by immunohistochemistry.Results:The proportion of spleen MDSC... |
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| Keywords: | Myeloid-derived suppressor cells Immune escape Tumor-bearing Hepatocarcinoma |
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