电针的不同波形对实验性大鼠急性痛风性关节炎滑膜组织中HSP70表达的影响

目的:明确不同波形电针治疗急性痛风性关节炎的疗效,探讨电针对实验性急性痛风性关节炎大鼠关节局部保护作用及其机制.方法:采用Coderre等经典造模方法建立急性痛风性关节炎大鼠模型.随机分成5组:空白组、模型组、电针疏波组、电针密波组、电针疏密波组.各组分成1天、3天、5天共3个时间点,选取“足三里”、“三阴交”穴进行电针治疗.以免疫组织化学技术观察病变关节滑膜组织中HSP70的表达变化,并进行统计学分析.结果:电针疏密波组能使急性痛风性关节炎大鼠滑膜内HSP-70蛋白表达显著增加(P＜0.05或P＜0.01),从而抑止滑膜组织炎症发生.结论:电针不同波形均能有效上调急性痛风性关节炎大鼠足踝关节滑膜内HSP70蛋白表达,从而减轻踝关节局部炎症反应,减轻关节损伤,且以电针疏密波为最佳.

The Effect of Electro Acupuncture in Different Waveforms to HSP70 Expression in Rats of Acute Gouty Arthritis

Objective：To clear up the therapeutic effects of electroacupuncture in different waveforms to acute gouty arthritis, and reveal its mechanisms of partial protection for acute gouty arthritis. Methods：Coderre % Classical methods was used to establish the acute gouty arthritis model for rats. Wistar rats were divided ran-domly into five groups ： blank group, model group, group of electroacupuncture in Shu - wave, group of elec- troacupuncture in dense - wave, group of electroacupuncture in Shu and dense wave. Each group was separated into 1 day,3 day, and 5 day as subgroup for investigation. Select ＂Zusanli＂, ＂Sanyinjiao＂ to be electroacupunc- ture points. To observe the changes of the expression of HSP70 in the diseased synovial tissue by using immuno- histochemical techniques and statistically analyzed the data. Results：Group of electroacupuncture in Shu and dense wave can significantly promote the expression of HSP - 70 protein in synovial tissues of acute gouty arthri- tis rats （ P 〈 0.05 or P 〈0. 01 ）, thus restrains the occurrence of synovial tissue inflammation. Conclusion： Electroacupuncture of different waveforms can significantly increase HSP - 70 protein expression, so as to reduce the local inflammatory response of ankle joint and joint damage in synovial tissues of acute gouty arthritis rats. The group of electroacupuncture in Shu and dense wave is better than others.