大鼠脑梗死后神经前体细胞移行及电针作用的研究

目的研究成年大鼠脑梗死后电针作用对内源性神经前体细胞移行的影响。方法采用易卒中型肾性高血压大鼠（RHRSP）,电凝法凝闭大脑中动脉（MCAO）。用Garcia评分、HE染色、免疫荧光等方法观察电针对脑梗死后1周、2周、3周、4周大鼠脑内梗死灶体积,行为学评分及5-溴脱氧尿核苷（Bromodeoxyuridine,Brdu）、Doublecortin（DCX）阳性细胞数的干预作用,并与脑梗死组和对照组比较。结果电针治疗后前3周梗死体积较脑梗死组缩小（P〈0.05）;电针后2d与3d大鼠行为学评分较梗死组高（P〈0.05）;MCAO后梗死灶边缘有Brdu阳性细胞和DCX阳性细胞分布。电针组Brdu阳性细胞较脑梗死组明显增加（P〈0.05）,电针组DCX阳性细胞数在1周末明显高于脑梗死组（P〈0.05）。结论电针促进神经功能恢复,减小脑梗死体积,并使缺血灶周移行的神经前体细胞增多,可能与其改变脑内微环境密切相关。

Effect of Electroacupuncture on Migration of Neural Precursor Cells after Acute Cerebral Infarction in Rats

Objective To investigate the effect of electroacupuncture（EA） on migration of neural precursor cells（NPCs） after acute cerebral infarction in rats.Methods Models of stroke-prone renovascular hypertensive rats（RHRSP） were established by middle cerebral artery occlusion（MCAO）.The effects of EA on the Bromodeoxyuridine（Brdu） positive cells and doublecortin（DCX） positive cells were detected by immunofluorescence.The infarction volume,ethology scores were compared with in control group and cerebral infarction group at week 1,2,3,and 4 after ischemic stroke.Results The infarct volume in EA group was reduced compared with that in cerebral infarction group after 3 weeks（P0.05）,the ethology scores in EA group were markedly higher than that in cerebral infarction group（P0.05）.The expression of the Brdu positive cells and DCX positive cells were increased in the ischemic area after MCAO.After EA,Brdu positive cells in the ischemic area were increased significantly than that in cerebral infarction group（P0.05）.Conclusion The EA may promote the neural functional recovery and reduce infarct volume.