| 银杏提取物对培养的脑微血管内皮细胞与单核细胞和中性粒细胞 … |
| |
| 摘 要： |
|
| 关 键 词： | 血管内皮 银杏 细胞粘附 单核细胞 E－选择素 |
Antagonistic effects of Ginkgo biloba extract on adhesion of monocytes and neutrophils to cultured cerebral microvascular endothelial cells. |
| |
| J P Xu,Y C Rui,T J Li. Antagonistic effects of Ginkgo biloba extract on adhesion of monocytes and neutrophils to cultured cerebral microvascular endothelial cells.[J]. Acta Pharmacologica Sinica, 1999, 20(5): 423-425 |
| |
| Authors: | J P Xu Y C Rui T J Li |
| |
| Affiliation: | Department of Pharmacology, College of Pharmacy, Second Military Medical University, Shanghai, China. |
| |
| Abstract: | AIM: To study the action of Ginkgo biloba extract (GbE) on tumor necrosis factor (TNF-alpha)-induced adhesion of monocytes (Mon) and neutrophils (Neu) to cultured cerebral microvascular endothelial cells. METHODS: TNF-alpha-induced endothelial adhesivity toward Mon and Neu was studied using bovine cerebral microvascular endothelial cells (BCMEC) in vitro. The number of Mon and Neu adhering to the BCMEC monolayers was determined by flow cytometry. RESULTS: Pretreatment of BCMEC with TNF-alpha increased Mon and Neu adhesion to BCMEC from 12.5% +/- 0.2% to 31.3% +/- 0.5% and from 13.8% +/- 0.4% to 32.1% +/- 0.5%, respectively. GbE (1-100 mg.L-1) inhibited the effect of TNF-alpha in a concentration-dependent manner. E-selectin mAb (1 mg.L-1) blocked Mon and Neu adhesion to BCMEC induced by TNF-alpha. CONCLUSION: The inhibition of GbE on Mon and Neu adhesion to BCMEC was mediated through the suppression of E-selection expression. |
| |
| Keywords: | |
|
|
