侵袭性牙周炎患者外周血CD-4+CD-25+调节性T细胞的检测及功能分析 |
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引用本文: | 赵川江,徐琛蓉,周玉竹,苏小鹏. 侵袭性牙周炎患者外周血CD-4+CD-25+调节性T细胞的检测及功能分析[J]. 中华口腔医学杂志, 2008, 44(1): 413-415. DOI: 10.3760/cma.j.issn.1002-0098.2009.07.008 |
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作者姓名: | 赵川江 徐琛蓉 周玉竹 苏小鹏 |
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作者单位: | 中山大学光华口腔医学院牙周科,广州,510055;广东省口腔医院牙周科; |
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摘 要: | Objective To investigate the changes of proportion and suppression function of CD-4+ CD-25+ regulatory T cells in the peripheral blood of patients with aggressive periodontitis.Methods Flow cytometric analysis was used to detect the frequency of CD-4+ CD-25+ regulatory T cells in the peripheral blood of 16 patients with generalized aggressive periodontitis and 17 patients with chronic periodontitis,as well as 17 periodontal healthy controls.Furthermore,CD-4+ CD-25+ regulatory T cells and CD-4+ CD-25- T cells were separated from peripheral blood of each enrolling subject using magnetic cell sorting technology.The direct suppression effect of CD-4+ CD-25+ regulatory T cells on CD-4+ CD-25- T lymphocytes proliferation was performed by the mixed lymphocytes reaction and measured by 3H-thymidine radioactive assay.Results The patients with generalized aggressive periodontitis had a lower frequency of CD-4+ CD-25+ regulatory T cells(9.71±4.01)%in the peripheral blood than periodontal healthy controls [(14.72±3.51)%]and chronic periodontitis patients[(17.01±5.16 )%],P<0.05.A significant decrease was found in the suppression function of CD-4+ CD-25+ regulatory T cells from peripheral blood of patients with generalized aggressive periodontitis when co-cultured with CD-4+ CD-25- T lymphocytes in the proportion of 2:1,1:1 and 1:2 as compared with chronic periodontitis patients and periodontal healthy controls(P<0.05).Conclusions Diminished numbers and decreased suppression function of CD-4+ CD-25+ regulatory T cells were found in patients with generalized aggressive periodontitis.
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关 键 词: | T淋巴细胞,调节性 流式细胞术 侵袭性牙周炎 |
Detection and functional analysis of CD-4+ CD-25+ regulatory T cells in the peripheral blood of patients with generalized aggressive periodontitis |
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Abstract: | |
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Keywords: | T-lymphocytes regulatoryFlow cytometryAggressive periodontitis |
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