抗CD81抗体对星形胶质细胞增殖的抑制作用

目的 研究抗CD81抗体在星形胶质细胞增殖中的作用.方法 将纯化的星形胶质细胞分为6组,加入不同浓度抗CD81抗体,其浓度依次为0、0.1mg/L、0.5mg/L、1mg/L、5mg/L、10mg/L,以四甲基噻唑蓝(MTT)比色法检测细胞活性.在此检测结果的基础上,选出3个有意义的浓度组,加入浓度分别为0、0.5mg/L、5mg/L的抗CD81抗体,采用流式细胞术观察抗CD81抗体对星形胶质细胞周期的影响并进行统计学分析.结果 抗CD81抗体对星形胶质细胞的增殖有抑制作用,并呈一定的剂量依赖性.加入抗CD81抗体培养24 h后,实验组的星形胶质细胞处于G0/G1期的细胞指数减少,S期细胞指数增多.结论 抗CD81抗体抑制了星形胶质细胞的增殖,使星形胶质细胞的细胞周期受阻,阻滞于S期.

THE INHIBITION EFFECT OF ANTI-CD81 ON THE PROLIFERATION OF ASTROCYTES

Objective To investigate the effect of anti CD81(antibodys against CD81) on the proliferation of astrocytes. Methods Purified astrocytes from newborn rats’ cerebral cortex were divided into 6 groups and added with anti CD81 different concentrations(0,0.1，0.5，1,5，10mg/L). The activity of astrocytes was tested by methyl thiazolyl terazolium(MTT). Three significative groups were chosen based on MTT result and added with anti CD81 of different concentrations(0,0.5，5mg/L). After administration for 24 hours, the cell cycle of the astrocytes was measured by flow cytometer. The corresponding data were analyzed with SPSS statistical software. Results 1By MTT, the average optical density(AOD) values of astrocytes were reduced after administration with anti CD81 of different concentrations for 24 hours, that is, the number of astrocytes was reduced, which indicated anti CD81 inhibited the proliferation of astrocytes and the effect showed a dose dependent pattern.2By cell cycle analysis, a progressive dose dependent decrease was found in the index of cells in G0/G1 phase and an increase in S phase. Such as, the index of cells in G0/G1 phase, was 82.73 in 0, is 8216 in 0.5mg/L, was 78.58 in 5mg/L. Conclusion Anti CD81 inhibits the proliferation of astrocytes and the number of astrocytes is reduced.