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| 弓形虫感染与生精细胞凋亡关系的研究 | |
| 引用本文： | 周永华,胡玉红,石火英,王瑞兵,陆永娟.弓形虫感染与生精细胞凋亡关系的研究[J].中国血吸虫病防治杂志,2003,15(4):285-287. |
| 作者姓名： | 周永华 胡玉红 石火英 王瑞兵 陆永娟 |
| 作者单位： | 1. 江苏省寄生虫病防治研究所,无锡,214064 2. 南京军区南京总医院 3. 扬州大学农学院 4. 江苏省苏州市吴中区计划生育局 |
| 基金项目： | 江苏省计生委基金(JS-2001-04) |
| 摘 要： | 目的 探讨弓形虫感染与生精细胞凋亡的关系。方法 采用瑞-姬染色和末端脱氧核苷酸转移酶(TdT)介导的脱氧核苷酸原位末端标记法(TUNEL)检测弓形虫感染小鼠精子悬液的凋亡生精细胞。结果 弓形虫感染组生精细胞凋亡率明显高于正常对照组(P<0.05)。结论 弓形虫感染可能有促进生精细胞凋亡的作用。 |
| 关 键 词： | 弓形虫感染 生精细胞凋亡 瑞-姬染色 原位未端标记法 |
| 文章编号： | 1005-6661(2003)04-0285-03 |
| 修稿时间： | 2003年1月3日 |
EXPERIMENTS ON RELATIONSHIP BETWEEN INFECTIONS OF TOXOPLASMA GONDII AND APOPTOSIS OF SPERMATOGENIC CELLS |
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| Zhou Yonghua,Hu Yuhong,Shi Huoying,Wang Rui bing,Lu Yongjuan.EXPERIMENTS ON RELATIONSHIP BETWEEN INFECTIONS OF TOXOPLASMA GONDII AND APOPTOSIS OF SPERMATOGENIC CELLS[J].Chinese Journal of Schistosomiasis Control,2003,15(4):285-287. | |
| Authors: | Zhou Yonghua Hu Yuhong Shi Huoying Wang Rui bing Lu Yongjuan |
| Abstract: | Objective To inquire into the relationship between infections of Toxoplasma gondii and apoptosis of spermatogenic cells. Methods Apoptotic spermatogenic cells of mice infected with Toxoplasma gondii were examined by Wright-Giemsa staining and terminal deoxynucleotidyl trans-Icrase (TdT) mediated deoxyuridine triphosphate (dUTP)-biotin nick-end labeling (TUNED techniques. Results Apoptosis rate of infective group of Toxoplasma was significantly higher than thai oi control group (F<0. 05). Conclusion Apoptosis of spermatogenic cells may be promoted by in fections of Toxoplasma gondii. |
| Keywords: | Infection of Toxoplasma gondii Apoplosis of spermatogenic cells Wright-Giemsa statning: Triphosphate (dUTP)-biotin nick-end labeling (TUNED technique |
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