胃癌中P-gp和LRP的表达及临床意义

目的:探讨P-糖蛋白(P-gp)和肺耐药蛋白(LRP)在胃癌中的表达及其临床意义。方法:应用免疫组化SABC法检测P-gp和LRP在80例胃癌组织和20例正常胃粘膜组织中的表达,探讨胃癌组织学类型、肿瘤浸润深度及有无淋巴结转移对P-gp和LRP表达的影响。结果:P-gp和LRP在胃癌组织中的阳性表达率高于正常胃粘膜组织(P<0.05);在高中分化腺癌中的表达显著高于低分化腺癌(P<0.05);在浸润粘膜及粘膜下层组、肌层组、浆膜及浆膜外组表达率无显著差别(P>0.05);在有淋巴结转移组P-gp、LRP表达率显著高于无淋巴结转移组(P<0.05)。结论:P-gp、LRP在胃癌原发性多药耐药中起重要作用,其表达与组织学类型、有无淋巴结转移有关而与肿瘤浸润深度无关,联合检测P-gp、LRP对于胃癌判断预后、制定化疗方案有一定的指导意义。

Clinical Significance and Expression of P-gp and LRP in Gastric Carcinoma

Objective: To study the clinical significance and expression of glutathione-s-trannsferase(P-gp) and lung resistance protein(LRP) in gastric carcinoma tissue.Method: The expression of P-gp and LRP in 80 cases of gastric carcinoma and 20 cases of normal stomach tissues was detected by SABC immunohistochemical technique,its correlation with clinical pathologic characteristic of gastric carcinoma,including histological type,invasion degree and lymph node metastasis, was explored.Result: The positive expression rates of P-gp and LRP in gastric carcinoma were all higher than those in normal stomach tissues(P <0.05).The expression rates of P-gp and LRP in well-moderated differentiation adenocarcinoma were significantly higher than those in poor differentiation adenocarcinoma(P<0.05).There was no significant differentiation between the expression rates of P-gp and LRP in the three groups invading mucoma and submocuma,invading musculairs and invading serosa and beyond serosa(P>0.05).The expression rates of P-gp and LRP in lymph metastasis were significantly higher than those in without lymph node metastasis(P<0.05).Conclusion: P-gpand LRP play an important roles in the primary MDR of gastric carcinoma.The expression of them is associated with histological type and lymph node metaaastasis,while not associated with invased layer.The determination of P-gp and LRP may be useful for the estimatation of prognosis and the establishment of chemical therapy regimen in gastric carcinoma.