龙胆草对人黑素细胞增殖及黑素合成的影响

目的探讨龙胆草对人黑素瘤细胞增殖及黑素合成的影响。方法采用MTT法测定龙胆草对人A375黑素瘤细胞增殖的影响，NaOH裂解法测定黑素合成，并与8-MOP的结果进行比较。结果0．1mg／mL、0．2mg／mL和0．5mg／mL龙胆草对人A375黑素瘤细胞增殖无影响，0．1mg／mL、0．2mg／mL和0．5mg／mL龙胆草对人A375黑素瘤细胞酪氨酸酶活性及黑素生成均有较强的剂量相关性激活作用，0．2mg／mL及0．5mg／mL龙胆草与对照组相比差异有统计学意义（P〈0．01）。结论龙胆草对培养的人黑素瘤细胞酪氨酸酶活性及黑素生成有较强的剂量相关性激活作用。

Effects of Radix Gentianae on Cell Proliferation and Melanin Synthesis of Cultured Human Melanoma Cells

Objective To study the effects of Radix Gentianae on cell proliferation and melanin synthesis of cultured human melanoma cells. Method MTF cytotoxicity assay and NaOH-assay were employed to measure the cell viability and melanin synthesis of human A375 melanoma cells; tyrosinase activity was measured by utilization of L-Dopa as the substrate. Furthermore, the effects of Radix Gentianae on human A375 melanoma cells were compared with 8-MOP. Result No influence was observed on the proliferation of human A375 cell after treatment with 0.1 mg/mL, 0.2 mg/mL, or 0.5 mg/mL of Radix Gentianae. The melanin synthesis and tyrosinase activity were clearly enhanced by Radix Gentianae in a dose-dependent manner at concentrations of 0.1 mg/mL, 0.2 mg/mL, and 0.5mg/mL. Ignificant enhancement was observed at the concentrations of 0.2 mg/mL and 0.5 mg/mL （P〈0.01）. Conclusion Radix Gentianae exhibits a dose-dependent enhancing effect on melanin synthesis and tyrosinase activity in human A375 melanoma cells.