TRAIL诱导乙型肝炎病毒转染细胞株凋亡的实验研究

目的 探讨人肿瘤坏死因子相关凋亡诱导配体（TNF-related apoptosis inducing ligand，TRAIL）对HBV稳定转染的肝癌细胞株HepG2．2．15的凋亡诱导效果。方法应用MTT法了解TRAIL对HepG2．2．15细胞的诱导效果，琼脂糖凝胶电泳、流式细胞术和Caspase．3酶活性分析TRAIL诱导HepG2．2．15细胞凋亡过程。结果TRAIL（0．1μg／mL）作用于HepG2．2．15细胞株12、24、36h后，细胞抑制率分别为17．91％、41．26％、59．85％；PI单染亚二倍体含量12h后为21．8％，高于对照组的8．7％；24h后DNA电泳200bp处出现特异性条带；6h后Caspase-3酶活性为对照组的4．76倍。结论TRAIL诱导后，HBV稳定转染的肝癌细胞株HepG2．2．15发生凋亡

Induction of Apoptosis in A Hepatitis B Virus Stablely Infected Cell Line by TRAIL

Objective To examine the induction of apoptosis of a HBV stably infected cell line HepG2.2.15 by TNF-related apoptosis-inducing ligand （TRAIL）. Methods MTT was used to determine the cytotoxic effect of TRAIL on the HepG2.2.15 cells. DNA electrophoresis, spectrophotometry and flow cytometry were used to characterize the apoptotic process of HepG2.2.15 ceils treated with TRAIL. Results The inhibition rate of TRAIL （0.1μg/mL） on HepG2.2.15 at 12, 24 and 36 hours was 17.91%, 41.26%, and 59.85%, respectively. The percentage of PI stained sub-G1 cells （at 12 hours） in the TRAIL-treated and control cell was 21.8% and 8.7%, respectively. Typical DNA ladder was observed in cells at 24 hours after TRIAL treatment. After 6 hours of the treatment, the activity of Caspase-3 was about 4.76 times higher than the untreated control cells. Conclusion TRAIL was found to induce apoptosis in the HepG2.2.15 cells