芯片电泳快速检测高危型人类乳头状瘤病毒

目的建立人类乳头状瘤病毒（humanpa pillomavims,HPV）52和58基因型高危型的芯片电泳检测方法。方法选取114例宫颈细胞检测患者,提取宫颈脱落细胞DNA。PCR扩增HPV高危型52和58基因型特异性DNA序列,应用优化的芯片电泳分析方法对扩增产物进行分离检测。根据细胞学诊断结果将研究对象分为4组：正常组、不典型鳞状细胞组、低度鳞状上皮内病变组和高度鳞状上皮内病变组,比较各组的HPV52、58基因型感染情况。结果114份标本中,HPV52基因型检出率为8.8%（10/114）,HPV58基因型检出率为33.3%（38/114）。与正常对照组相比较,各病变组HPV52基因型检出率差异有统计学意义（P〈0.05）;而HPV58基因型检出率在病变组和正常对照组之间差异无统计学意义（P〉0.05）。结论芯片电泳法检测快速、成本低,可用于HPV52、58基因型感染的筛查。

Fast Detection of the High Risk Human Papillomaviruses Using Microchip Electrophoresis

Objective A microchip electrophoresis based method was developed for the detection of high risk human papillomaviruses （HPV）. Methods 114 cervical samples were classified into 4 groups according to their histological diagnosis as normal cytological diagnosis, unknown significance （ASCUS）, low-grade intraepithelial lesions （LSIL）, and high-grade intraepithelial lesions （HSIL）. The extracted DNA samples were amplified with HPV 52 and 58 specific primers. The PCR amplicons were analyzed with an optimized microchip electrophoresis method. The correlation between genotype 52, 58 distributions of HPV and the cytological group classifications for 114 cervical samples was examined. Results The total infection rates of HPV 52 and 58 detected by microchip electrophoresis were 8.8% and 33.3%, respectively. There was statistical significant difference in positive rate of HPV 52 between the normal cytology group and each of the other three groups （P〈0.05）, while no significant differences were fonnd in HPV58. Conclusion The microchip electrophoresis based HPV genotyping assay is fast and low-cost method, thus well suited to clinical screening of high risk HPV.