芍药苷对脑缺血损伤的保护作用及其对脑血管通透性的影响

目的观察芍药苷（peoniflorin，PF）注射液对脑缺血损伤的保护作用及其对脑血管通透性的影响。方法采用线栓法闭塞大脑中动脉建立大鼠局灶性脑缺血模型（MCAO），各组分别进行行为学评分，脑组织含水量和脑梗塞面积测定及免疫组化检测脑组织中水通道蛋白-4（AQP4）的表达。此外，检测PF对脑缺血再灌注损伤小鼠脑血管通透性的影响。结果PF注射给药可显著改善脑缺血的神经损伤症状，降低脑组织的含水量，缩小脑梗死面积，降低脑血管通透性，减少AQP4阳性细胞的表达。结论芍药苷注射液对脑缺血有明显的保护作用，该作用可通过调节AQP4的表达而降低脑血管通透性来完成。

Protective Effects of Peoniflorin on Cerebral Ischemia Injury and Its Effects to Cerebral Vessels Permeability

Objective To study the protective effects of peoniflorin （PF） on cerebral ischemia injury and its effects to cerebral vessels permeability. Method Ethological score, water content of brain tissue, cerebral infarct size and the express of aquaporion-4 （AQP4） of the middle cerebral artery occlusion （MCAO） rats were used to determine the effect of PF. Effects of PF on brain capillary permeability was determined in global cerebral ischemia-reperfusion mice. Result PF decreases ethological score, water content and infraction zone of brain. It reduces brain capillary permeability and induced the expression of AQP4. Conclusion PF has obvious protective effects to brain ischemia, which may be due to the regulation of AQP4 and decrease of brain capillary permeability.