手足口病患儿T淋巴细胞亚群与CD3^＋CD4ˉCD8ˉ双阴性调节性T细胞的检测及其临床意义

目的探讨外周血T淋巴细胞亚群及CD3^＋CD4ˉCD8ˉ双阴性调节性T细胞在手足口病病人发病中的临床意义。方法采用流式细胞术检测了46例手足口病患者以及17例健康体检者外周血T细胞亚群CD3^＋、CD3^＋CD4ˉCD8^＋、CD3^＋CD4^＋CD8^＋、CD3^＋CD4^＋CD8ˉ与CD3^＋CD4ˉCD8ˉ双阴性调节性T细胞水平。结果与正常对照组相比，手足口病患者组CD3^＋、CD3^＋CD4ˉCD8’、CD3^＋CD4ˉCD8ˉ双阴性调节性T细胞的百分率降低（P值分别为0．008、0．050、0．025）。CD3^＋CD4^＋CD8^＋、CD3^＋CD4^＋CD8ˉ百分率以及CD4／CD8比值差异无统计学意义（P值分别为0．847、0．775、0．149），以上各细胞亚群的百分率以及CD4／CD8比值在年龄与性别方面差异无统计学意义。结论手足口病患者CD3^＋CD4ˉCD8ˉ双阴性调节性T细胞、CD3^＋、CD3^＋CD4ˉCD8^＋异常变化提示其免疫功能异常。

Clinical Significance of T-cell Subsets and Double-Negative Regulatory T cells in Hand Foot Mouth Disease

Objective To determine the percentage of T-cell subsets and the double-negative regulatory T cells in peripheral blood of hand foot mouth disease （HFMD） patients and the clinical significance of these T cell subsets. Methods The percentage of T-cell subsets and the double-negative regulatory T cells of CD3^＋, CD3^＋CD4ˉ CD8^＋, CD3^＋CD4^＋CD8^＋, CD3^＋CD4^＋CD8ˉ and CD3^＋CD4ˉCD8ˉ in the peripheral blood of 46 patients with HFMD and 17 control subjects were determined by flow cytometry. Results Compared with the control subjects, the percentage of CD3^＋, CD3^＋CD4ˉCD8^＋ and CD3^＋CD4ˉCD8ˉ in patients with HFMD was significantly decreased （P 〈 0.05）. The percentage of CD3^＋CD4^＋CD8^＋, CD3^＋CD4^＋CD8ˉ and the ratio of CD4/CD8 were not significantly changed （P 〉 0.05）. There was no sex and age difference in the percentage of T-cell subsets in the HFMD patients. Conclusion The immunologic function of HFMD patients was abnormal.