Prenatal urinary matrix metalloproteinase profiling as a potential diagnostic tool in fetal obstructive uropathy |
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Authors: | Grace A Nicksa Adam S Curatolo Carol E Barnewolt Terry L Buchmiller Dario O Fauza |
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Institution: | a Department of Surgery, Children's Hospital Boston & Harvard Medical School, Boston, MA 02115, USA b Vascular Biology Program, Children's Hospital Boston & Harvard Medical School, Boston, MA 02115, USA c Department of Radiology, Children's Hospital Boston & Harvard Medical School, Boston, MA 02115, USA |
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Abstract: | Background/PurposeThe diagnostic evaluation, patient stratification, and prenatal counseling for congenital obstructive uropathy remain sub-optimal. Matrix metalloproteinase (MMP) expression profiles are emerging as a valuable diagnostic tool in assorted disease processes. We sought to determine whether congenital obstructive uropathy impacts MMP expression in fetal urine.MethodsFetal lambs (n = 25) were divided in two groups: group I (n = 12) underwent a sham operation and group II (n = 13) underwent creation of a complete urinary tract obstruction. Gelatin zymography panels for 4 MMP species were performed on fetal urine in both groups at comparable times post-operatively. Statistical analysis was by the Fisher's exact test (P < .05).ResultsOverall fetal survival was 80% (20/25). A variety of significant differences in MMP expression between the two groups were identified. The following profiles were present only in obstructed animals: any MMP other than MMP-2 (P = .029), including any MMP other than 63 kDa and 65 kDa (P = .009); 2 or more MMPs excluding MMP-2s (0.029); and 3 or more MMPs (P = .029).ConclusionsLimited matrix metalloproteinase expression is present in the urine of normal ovine fetuses. Fetal obstructive uropathy impacts urinary MMP expression in various distinguishable patterns. Prenatal urinary MMP profiling may become a practical and valuable diagnostic tool in the evaluation of congenital obstructive uropathy. |
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Keywords: | Matrix metalloproteinase Fetus Obstructive uropathy Urine Prenatal diagnosis |
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