斑点金免疫渗滤法检测血清抗精子抗体

目的建立检测血清抗精子抗体的斑点金免疫渗滤法 (dot im munogold filtration assay,DIGFA)。方法　采用经 Sephadex G- 75 -抗人全血清柱亲和层析纯化的人精子抗原和胶体金标记的 SPA,根据免疫渗滤原理 ,建立抗精子抗体(ASAb)的斑点金免疫渗滤法检测法。结果　纯化的人精子抗原具有较好的特异性 ;斑点金免疫渗滤法与 EL ISA法比较 ,符合率为 97.7%。特异性为 96 .2 % (TN/ TN+ FP× 10 0 % ) ,敏感性为 88.9% (TP/ TP+ FN× 10 0 % ) ,阳性预测值 84.2 % (TP/TP+ FP) ,阴性预测值 97.2 % (TN/ TN+ FN) .结论　斑点金免疫渗滤法方法检测血清抗精子抗体有较好的特异性和敏感性 ,重复性好 ,试剂稳定 ,操作简便快速 ,具有临床应用价值

Dot immunogold filtration assay for detection of anti sperm antibody in serum of infertile couples

Objective Using dot immunogold filtration assay（DIGFA） toestablish a new method for detecting anti-sperm antibody in serum of infertile couples.Methods A dot immunogold filtration assay was developed by coating purified sperm membrane antigen on nitrocellulose membrane， labeling SPA with colloidal gold， the whole procedure could be finished in 2-4 min.Results The coincidence rate of the results of simultaneous determination of serum samples with ELISA and DIGFA was 97.7％.DIGFA had an overall specificity and sensitivity of 96.2％（TN／TN＋FP×100％） and 88.9％（TP／TP＋FN×100％），positive predictive value and negative predictive value of 84.2％ and 97.2％ respectively.Conclusions Using DIGFA to detect anti-sperm antibody in human serum is more simple and rapid， and is of value for clinical application.