不同浓度甘草甜素对肝星状细胞增殖的影响及其调控机制的研究

目的探讨不同浓度甘草甜素对肝星状细胞增殖的影响及其调控机制的研究。方法用MTY法检测三组不同浓度甘草甜素对肝星状细胞增殖的抑制率，用免疫细胞化学法检测细胞周期素cyclinE的表达情况。结果MTT法检测不同药物浓度组：空白对照组、0．8mg／L甘草甜素组、1．2mg／L甘草甜素组、1．6mg／L甘草甜素组细胞增殖抑制率分别为0，2．73％，14．75％，25．96％，差异有统计学意义（P〈0．05）；不同浓度甘草甜素组中cyclinE阳性表达细胞数与对照组比较明显减少，且呈浓度依赖性，差异有统计学意义（P〈0．01）。结论甘草甜素能够浓度依赖性的抑制大鼠肝星状细胞增殖；甘草甜素可通过降低细胞周期素cyclinE的表达从而抑制大鼠肝星状细胞增殖。’

Different concentrations of glycyrrhizin on hepatic stellate cell proliferation and its control mechanism

Objective To explore the different concentrations of glycyrrhizin on hepatic stellate cell proliferation and its regulation mechanism.Methods Hepatic stellate cell proliferation rate of three different concentrations of glycyrrhizin was detected by MTT assay.The expression of cyclin E was detected by immunocytochemistry.Results MTT assay was used to detect the cell proliferation inhabitation in different concentration groups,from control group 0.8 mg/L glycyrrhizin group to 1.2 mg/L glycyrrhizin group and 1.6 mg/L glycyrrhizin group.The cell proliferation inhibited rates were 0,2.73%,14.75 % and 25.96%.The difference was significant (P＜0.05).The numbers of Cyclin E positive cells in different concentrations of glycyrrhizin group were significantly different compared with control group,and it was concentration dependently decreased (P＜0.01).Conclusions Glycyrrhizin concentration dependently inhibited rat hepatic stellate cells proliferation and it could reduce the expression of cyclin E and inhibit the proliferation of rat hepatic stellate cells.