大鼠睾丸中轻稀土元素含量及其分布特征研究

目的研究轻稀土元素La、Ce、Pr、Nd、Sm、Eu、Gd在大鼠睾丸中的吸收规律和蓄积特点。方法将雄性SD大鼠随机分为空白、对照、低、中、高及排毒组,每组10只。饲喂柠檬酸稀土28天后处死前5组,采取睾丸;排毒组在停止饲喂柠檬酸稀土的条件下再自由饲喂28天后处死,采取睾丸。用ICP-MS测定睾丸中上述稀土元素含量并做统计学分析。结果1.低、中、高剂量组大鼠睾丸中轻稀土元素含量逐渐增高,排毒组显著低于高剂量组;2.La、Ce、Pr在睾丸中分布符合奥多-哈尔金斯规则,Nd呈负偏离;3.多数轻稀土元素在睾丸和毛发中含量相关系数较高。结论轻稀土元素在睾丸中蓄积存在量效关系,分布有其自身特征,蓄积具有可逆性;轻稀土元素在毛发中的含量比其在全血中的含量更适合作为生物标志物。

Study on the characteristics of content and distribution of light rare earth elements in rat testes

OBJECTIVE: To study the characteristics of content and distribution of light Rare Earth Elements (LREEs) in rat testes. METHODS: Based on animal weight, 60 healthy male SD rats were randomly divided into two control groups and four experimental groups. After four week treat, the testes were collected except High-II . The High- II group was freely fed for another four week without the Citrate REEs. The LREE concentrations in the testes were determined by the inductively coupled plasma mass spectrometer (ICP-MS). RESULTS: (1) The concentrations of LREEs in testes increased with the increase of the doses. The concentrations of LREEs of High-II were much lower than those of High-I significantly (P < 0.05). (2) The distribution patterns of La, Ce, Pr in the testes approximated to the Odd Harkin's rule, but the Nd was an exception (negative deviation). 3. The correlation coefficients of LREEs concentrations between hair and testis were about 0.4, while those between blood and testis were much lower and have no significance. CONCLUSION: (1) Dose response relationship could exist in the accumulation of LREEs in the rat testes. (2) Testis could have its own distribution pattern of LREEs. (3) The accumulation of LREEs in testis could be reversible. (4) As it was, a biomarker of the LREEs in hair could be much better than that of blood.