| 利用TCR V_β基因分析GVHD患者的克隆性T细胞 |
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| 引用本文： | 杜欣,李扬秋,陆泽生,翁建宇,苏建华,林伟,黄梓伦. 利用TCR V_β基因分析GVHD患者的克隆性T细胞[J]. 现代免疫学, 2001, 21(1): 36-37,43 |
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| 作者姓名： | 杜欣 李扬秋 陆泽生 翁建宇 苏建华 林伟 黄梓伦 |
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| 作者单位： | 1. 广东省人民医院血液科,
2. 暨南大学血液病研究室, |
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| 基金项目： | 广东省1998年重点攻关项目；广东省卫生厅科学基金资助项目(A19017) |
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| 摘 要： | 应用RT PCR扩增 3例异基因造血干细胞移植术后合并慢性GVHD患者的外周血单个核细胞的TCRVβ 2 4个亚家族的CDR3,了解患者TCRVβT细胞的表达 ,PCR产物进一步经基因扫描分析确定T细胞克隆性。结果显示 ,3例患者均有克隆性T细胞生长 ,它可能与GVHD的发生有关。
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| 关 键 词： | TCRV_β基因 基因扫描 T细胞克隆性 移植物抗宿主病 |
| 文章编号： | 1001-2478(2001)01-0036-02 |
Evidence for Oligoclonal TCell Expamion in Chronic Graft-Versus-Host-Disease Cases |
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| DU Xin,LI Yang-qiu,LU Zhe-sheng,WENG Jian-yu,SU Jian-hua,LIN Wei,HUANG Zi-lun. Evidence for Oligoclonal TCell Expamion in Chronic Graft-Versus-Host-Disease Cases[J]. Current Immunology, 2001, 21(1): 36-37,43 |
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| Authors: | DU Xin LI Yang-qiu LU Zhe-sheng WENG Jian-yu SU Jian-hua LIN Wei HUANG Zi-lun |
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| Affiliation: | DU Xin 1,LI Yang qiu 2,LU Zhe sheng 1,WENG Jian yu 1,SU Jian hua 1,LIN Wei 1,HUANG Zi lun 1 |
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| Abstract: | Complementarity determining region 3 (CDR3)of TCR Vβ with 24 variable region gene was amplified in peripheral blood mononuclear cells drawn from three cases of chronic graft-versus-host disease after allogenic stem cell transplantation. The RT-PCR was used to observe the expression of TCR Vβ repertoire T cells and the PCR products were further analyzed by genescan to evaluate clonality of T cell. The results showed that 2 or 7 TCR Vβ subfamily T cells were expressed in each sample of chronic GVHD cases. Oligoclonal T cells were identified in TCR Vβ 2, 3, 8, and 17repertoire. These results indicate that clonal expansion of T cells could be found in chronic GVHD cases and might be related with the initiation of chronic GVHD. |
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| Keywords: | TCR Vβ gene genescan T cell clonality GVHD |
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