黄连解毒汤对糖尿病大鼠肠道二糖酶活性的影响

目的研究黄连解毒汤对糖尿病大鼠的降血糖作用及对糖尿病大鼠肠道二糖酶活性的影响。方法链脲佐菌素(ip)建立糖尿病大鼠模型;糖尿病大鼠ig给予黄连解毒汤提取物治疗1个月,观察治疗后血糖变化,测定各肠段肠内容物中的二糖酶(蔗糖酶、麦芽糖酶、乳糖酶)活性。正常大鼠小肠注入麦芽糖,30、60、120 min后取门静脉、外周静脉血,测定门静脉与外周静脉血中葡萄糖浓度差值。结果高、低剂量黄连解毒汤治疗后糖尿病大鼠的血糖分别降低(42.2±4.9)%、(23.1±3.8)%;黄连解毒汤治疗后糖尿病大鼠十二指肠、空肠和回肠的麦芽糖酶活性分别降低(32.2±4.3)%、(26.5±2.9)%、(36.5±5.2)%;蔗糖酶活性分别降低(33.3±3.7)%、(32.8±4.6)%、(27.4±3.3)%,乳糖酶活性分别降低(35.4±4.2)%、(17.9±2.3)%、(6.3±1.8)%。黄连解毒汤能减小大鼠小肠注入麦芽糖后门静脉与外周静脉血中葡萄糖浓度差值。结论黄连解毒汤能显著降低糖尿病大鼠血糖,其降糖机制可能与抑制糖尿病大鼠肠道二糖酶活性有关。

Effects of Huanglian Jiedu Decoction on activities of intestinal disaccharidases in diabetic rats

Objective To investigate the hypoglycemic activity of Huanglian Jiedu Decoction(HJD) and its effects on the activities of small intestine disaccharidases in diabetic rats induced by Streptozotocin(STZ).Methods The diabetic rats were made by ip injection of STZ.Then the diabetic rats were treated by ig administration of HJD for one month.The level of plasma glucose was examined after the treatment by HJD.When the treatment finished,rats were killed and the intestinal contents were collected for the determination of disaccharidases(sucrase,maltase,and lactase) activities.Moreover,blood samples from portal and peripheral veins were separately collected at 30,60,and 120 min after maltose solution was infused into small intestine in vivo,and then the differences of blood glucose concentration between portal and peripheral veins were determined.Results After the treatment of two dosage of HJD,the level of plasma glucose in diabetic rats was significantly decreased by(42.2±4.9)% and (23.1±3.8)%,respectively.And when compared with diabetic group,the activities of maltase in the duodenum,jejunum,and ileum of diabetic rats treated with HJD were decreased by(32.2±4.3)%,(26.5±2.9)%,and(36.5±5.2)%,respectively. The activities of sucrase in the three intestinal sections of rats treated by HJD were decreased by(33.3±3.7)%,(32.8±4.6)%,and(27.4±3.3)%,respectively. And the acti-vites of lactase in the three intestinal sections were decreased by(35.4±4.2)%,(17.9±2.3)%,and(6.3±1.8)%,respectively.Furthermore,the difference of blood glucose concentration between portal and peripheral veins was also reduced after the treatment by HJD.Conclusion The results indicate that HJD possesses remarkably hypoglycemic effect on diabetic rats induced by STZ.And its preliminary mechanism may result from the inhibitory effects to the activities of intestinal disaccharidases.