肝胃乙醇脱氢酶活性变化与酒精性肝病发生的关系

目的 探讨肝、胃乙醇脱氢酶（ADH）活性的变化在酒精性肝病（ALD）不同病理阶段中的作用。方法 Wistar大鼠39只随机分成两组：模型组24只，对照组15只，用乙醇直接灌胃的方法建立大鼠ALD模型；用酶组织细胞化学染色技术，观察ALD不同病理阶段肝、胃ADH活性变化，并用LUZEX－F灰度图像分析仪进行ADH半定量。结果 ALD不同病理阶段肝ADH活性进行性增加，胃ADH活性进行性下降，两者均与对照组比较差异有显著性（P＜0．05）。结论 肝、胃ADH活性变化可能在ALD发生发展中起重要作用。

Relation between activities of hepatic and gastric alcohol dehydrogenases and formation of chronic alcoholic liver diseases

Objective To investigate the role of hepatic and gastric alcohol dehydrogenases (ADH) in different pathologic stages of alcoholic liver disease (ALD). Methods Thirty-nine rats were divided into either model or control group at random. The ALD model was established by infusing alcohol into the stomach. The activity varieties of hepatic and gastric ADH were observed by optical microscopy after hepatic and gastric tissues had been stained by enzyme histo-cytochemistry assay and the activity alterations were also determined with a LUZEX-F analysis instrument as a semi-quantitative method. Results The activity of hepatic ADH gradually increased, but gastric ADH gradually decreased in ALD. There was a significant difference between model group and control one (P<0.05). Conclusions The activity alterations of the hepatic and gastric ADH may play an important role in the onset and development of ALD. [