AG490单用或联合顺铂对人宫颈癌裸鼠移植瘤STAT3/Survivin信号转导通路的影响

目的:探讨STAT3信号转导通路对人宫颈癌裸鼠移植瘤生长、凋亡的影响以及与Survivin表达之间的关系。方法:建立宫颈癌裸鼠移植瘤模型并分组,AG490单用或联合顺铂(DDP)处理各组裸鼠,治疗期间观察并记录各组裸鼠皮下移植瘤的生长情况,计算肿瘤体积、肿瘤生长抑制率,绘制肿瘤生长曲线,观察用药后裸鼠体重变化;免疫组化、Western blot分别检测移植瘤P-STAT3、Survivin表达,流式细胞技术检测细胞凋亡。结果:AG490与DDP可以抑制宫颈癌移植瘤生长,促进宫颈癌细胞凋亡,同时使宫颈癌细胞P-STAT3、Survivin表达与活性明显下降;联合应用AG490与DDP,可以起协同作用,AG490可以增强DDP的药物敏感性并且减轻药物副作用。结论:JAK激酶抑制剂AG490与DDP联合应用可能为治疗宫颈癌提供了新的理论基础。

Effects of AG490 alone or AG490 combined with cisplatin on STAT3/Survivin signal transduction pathway in transplanted tumor of nude mice with human cervical cancer

Objective:To explore the effect of STAT3 signal transduction pathway on the growth and apoptosis of transplanted tumor of nude mice with human cervical cancer and its relationship with the expression of Survivin. Methods:The models of transplanted tumor of nude mice with human cervical cancer were established,then the mice were divided into different groups,AG490 alone or AG490 combined with cisplatin were used to treat the nude mice in different groups,the growth situations of subcutaneous transplanted tum...