HPLC测定石榴健胃丸中桂皮醛和胡椒碱的含量

目的建立高效液相色谱法同时测定石榴健胃丸中桂皮醛、胡椒碱含量的方法。方法色谱柱采用CAPCELL PAKC18(4.6 mm×250 mm,5μm)柱;流动相为乙腈-水(40∶60);流速:1.0 mL.min-1;检测波长:290 nm;柱温:30℃。结果桂皮醛在1.149 5～13.793 5μg之间呈良好的线性关系,平均回收率为97.27%,RSD为1.39%(n=6);胡椒碱在0.726 4～2.724μg之间呈良好的线性关系,平均回收率为96.51%,RSD为1.39%。结论本法简便、准确,专属性强,可作为控制石榴健胃丸质量的方法。

Determination of Cinnamaldehyde and Piperine in Shiliu Jianwei Pill by HPLC

OBJECTIVE To determine the content of Cinnamaldehyde and Piperine in Shiliu jianwei Pill. METHODS The analysis was performed on a CAPCELL PAK C18 Column（4.6 mm×250 mm, 5 um）at 30℃ with acetonitrile-water （40 ： 60）as the mobile phase and the flow rate of 1.0 mL.min-1. The detection wavelength was 290nm. RESULITS Cinnamaldehyde have good linearity in the range of 1.149 5-13.793 5 ug（r=0.999 7）. The average recovery was 97.27% and RSD was 1.39% （n=6）. Piperine have good linearity in the range of 0.726 4-2.724 ug, （r=0.999 1） respectively. The average recovery was 96.51% and RSD was 1.39% （n=6）. CONCLUSION The established method is accurate, reliable and specific. The quality of the pill can be controlled by the method.