| 人乳腺癌MCF-7细胞的TRF2 siRNA表达载体的构建和鉴定 |
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| 引用本文： | 陈绍坤,余红,刘岚,税青林,赵小平,黄燕. 人乳腺癌MCF-7细胞的TRF2 siRNA表达载体的构建和鉴定[J]. 中国妇幼保健, 2011, 26(15): 2344-2347 |
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| 作者姓名： | 陈绍坤 余红 刘岚 税青林 赵小平 黄燕 |
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| 作者单位： | 泸州医学院医学遗传学教研室,四川泸州,646000 |
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| 基金项目： | 四川省教育厅自然科学基金重点资助项目(2006A054) |
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| 摘 要： | 目的:构建表达端粒重复序列结合因子2(TRF2)基因小干扰RNA(siRNA-TRF2)的腺病毒表达载体(rAd-shRNA-TRF2),并检测该载体对人乳腺癌(MCF-7)细胞TRF2基因表达的沉默效应。方法:采用同源重组法构建重组腺病毒rAd-shRNA-TRF2表达载体,鉴定正确后转染MCF-7细胞,用FQ-RT-PCR和Western blot检测转染后48 h TRF2基因的表达,MTT法和克隆形成实验检测细胞生长状况。结果:重组腺病毒rAd-shRNA-TRF2包装成功,转染MCF-7细胞后,TRF2基因的表达明显被抑制,细胞生长显著抑制,细胞克隆形成能力显著下降。结论:腺病毒介导的TRF2 RNAi表达载体rAd-shRNA-TRF2构建成功,可有效抑制人乳腺癌MCF-7细胞TRF2基因的表达,抑制细胞生长。
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| 关 键 词： | TRF2 siRNA 腺病毒 肿瘤 基因治疗 |
Construction and identification TRF2 siRNA expression vector on the breast cancer MCF-7 cells |
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| CHEN Shao-Kun,YU Hong,LIU Lan,et al.. Construction and identification TRF2 siRNA expression vector on the breast cancer MCF-7 cells[J]. Maternal and Child Health Care of China, 2011, 26(15): 2344-2347 |
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| Authors: | CHEN Shao-Kun YU Hong LIU Lan et al. |
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| Affiliation: | CHEN Shao-Kun,YU Hong,LIU Lan,et al.Department of Medical Genetics,Luzhou Medical College,Luzhou 646000,Sichuan,China |
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| Abstract: | Objective:To construction adenovirus-mediated RNA interference vector expressing TRF2 small interfering RNA(rAd-shRNA-TRF2),and explore the specific inhibitory effect of this vector on TRF2 mRNA expression in MCF-7 cell.Methods:The recombinant adenovirus rAd-shRNA-TRF2 was obtained,and the vector was transfected into MCF-7 cells,then the expression of TRF2 mRNA and protein at 48 h after transfection was detected by FQ-RT-PCR and Western blot,the cell growth was detected by MTT colorimetry and clone forming ... |
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| Keywords: | TRF2 siRNA Adenovirus Tumor Gene therapy |
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