兔耳增生性瘢痕中Th1、Th2细胞相关趋化因子的表达及意义

目的研究辅助性T淋巴（Yh）细胞亚群Th1、Th2细胞相关趋化因子CXCL0／IP-10、CXCL12／SDF-1，CC12／MCP-1、CC1-3／MIP-1d、CCL5／RANTES、CCLT／MCP-3在兔耳增生性瘢痕形成过程中的表达变化及意义。方法选取14只新西兰大耳白兔，制作兔耳增生性瘢痕模型及兔背部正常性瘢痕模型。于术后第21,28、35,42,49、56、63天空气栓塞法分别随机处死2只兔子，采集瘢痕标本，行HE染色，测量并计算瘢痕增生指数；行Real—timePCR检测CXCL10、CXCL12、CC12、CCL3、CC15、CCL7的表达。结果HE染色可见兔耳增生性瘢痕组、兔背部正常性瘢痕组类似人增生性瘢痕、正常性瘢痕的镜下表现。瘢痕增生指数显示，THS组于术后第28天增生程度达到高峰（P〈0．05）。Real-timePCR结果示，Th2细胞相关趋化因子CC12、CCL-3、CCL5、CCL7、CCL13在兔背部正常性瘢痕组基本无表达，而在兔耳增生性瘢痕组存在长时间高表达（P〈0．05），Th1细胞相关趋化因子CXCL10、CXCL12在兔耳增生性瘢痕组长时间高表达，而在兔耳增生性瘢痕组处于低表达或不表达（P〈0．05）。结论在兔耳瘢痕增生过程中Th2细胞相关趋化因子cc也、CCL3、CCL5、CCL7存在长时间的高表达，Th1细胞相关趋化因子CXCL10、CXCL12低表达或无表达，提示瘢痕局部趋化因子的表达水平可能对Th1、Th2细胞在增生性瘢痕中的差异性表达起作用。

Expression of Th1/Th2 related chemokines in rabbit ear hypertrophic scar model

Objective To investigate the expression of Thl/Th2 related chemokines, CXCL0/IP-10, CX- CL12/SDF-1, CCI_2/MCP-1, CCL3/MIP-lot, CCL5/RANTES and CCL7/MCP-3 in rabbit ear hypertrophic scar. Methods Fourteen New Zealand white rabbits were selected to publish the hypertrophic scar （rHS） and normal scar （rNS） models on the ear and the back respectively. All the tissue specimens were harvested at 21,28, 35, 42, 49, 56 and 63 days after operation. Two rabbits were killed randomly by air embolism. Hematoxylin eosin staining was performed after fixing to observe morphological differences and then to measure SEI. The expression of CXCL10, CXCL12, CCL2, CCL3, CCI_5 and CCL7 was detected by RT-PCR. Results The sections of HE showed the micro- scopic structures of rabbit hypertrophic scar and normal scars like that of human scares. The SEI in the rHS group showed the degree of proliferation peaked at 28 days. The mR_NA level of Th2 cell related chemokines CC1.2, CCL3, CCI.5 and CCL7 were significantly higher in the rHS group while almost no expression in the rNS group. The mRNA level of Thl cell related chemokines CXCL10 and CXCL12 in the rHS group had no expression while was significantly higher in the rNS group （P〈0.05）. Conclusion Compared with the rNS group, the over-expression of Th2 cell related chemokine CCL2, CCI-3, CCL5 and CCL7 and the low-expression of Thl cell related chemokine CXCL10 and CXCL12 in the rHS group suggests that the level of ehemokines may play an important role in the pathogenesis and in Th1/Th2 cell distribution of hypertrophic scar.