关节腔注射青藤碱对兔膝骨性关节炎血清NO等自由基代谢的影响

目的 观察关节腔内注射青藤碱（SIN）对兔膝骨性关节炎（OA）血清中自由基代谢的影响,以探讨其防治OA的作用机理.方法 将38只兔随机分为正常组（A）、模型组（B）、透明质酸钠组（C）和青藤碱组（D）.A组不予任何处理,B,C和D三组通过关节腔注射木瓜酶法诱导OA模型后,分别用生理盐水、透明质酸钠和青藤碱干预,5周后检测并比较各组血清中一氧化氮（NO）、超氧化物歧化酶（SOD）和丙二醛（MDA）的含量.结果 B组血清NO和MDA含量分别为75.27±2.08 μmol/L和3.54±0.17 nmol/ml,均明显高于A组62.64±3.33 μmol/L和2.88±0.14 nmol/ml,差异有统计学意义（P值均＜0.01）;而B组SOD含量205.74±22.08 U/ml明显低于A组267.59±17.09 U/ml,差异有统计学意义（P＜0.01）.D组血清NO和MDA含量分别为69.29±1.51 μmol/L和3.09±0.16 nmol/ml,均明显低于B组,差异有统计学意义（P值均＜0.01）,低于C组72.69±1.84 μmol/L和3.33±0.22 nmol/ml,差异有统计学意义（P值均＜0.05）;而SOD含量245.46±11.25 U/ml,明显高于B组,差异有统计学意义（P＜0.01）,高于C组224.03±9.90 U/ml,差异有统计学意义（P＜0.05）.结论 关节腔注射青藤碱可以降低血清中NO,MDA含量,提高SOD活性,从而保护关节软骨,对OA起到防治作用.

Effect of Inject Sinomenine to Articular on the Metabolism of NO and Other Free Radicals in the Serum of Rabbits with Genicular Osteoarthritis

Objective To observe the effect of intra-artieular injection of sinomenine（SIN） on the metabolism of free radicalsin the serum of rabbits with genicular osteoarthritis（OA）,in order to investigate the mechanism of its prevention and treat-ment of OA. Methods The 38 rabbits were randomly divided into 4 groups：normal group（A） ,model group（B）, sodium hya-luronate group（C） and sinomenine group（D）. B,C, D group were established genicular osteoarthritis model by intra-articularinjectionof papain enzyme and were treated with saline,sodium hyaluronate and sinomenine respectively except for A group.5 weeks after treatment,investigate the level of the NO, SOD and MDA in the serum of the rabbits. Results The level ofNO （75.27±2.08） μmol/L and MDA （3.54±0.17） nmol/ml in B group were both higher than that of the A group （62.64±3.33） μmol/L and （2.88±0. 14） nmol/ml,there were distinct difference（both P〈0.01） ,while the level of SOD （205.74±22.08） U/ml was significantly lower than that of the A group （267.59± 17.09） U/ml （P〈0.01）. The level of NO（69.29±1.51） μmol/L and MDA（3. 09±0.16） nmol/ml in D group were both significantly lower than that of the B group（both P〈0.01） ,distinct lower than C group （72.69±1.84） tzmol/L and （3.33±0.22） nmol/ml （both P〈0.05） ,whilethe level of SOD （245.46±11.25） U/ml was significantly higher than that of the B group （P〈0.01） ,and distinct higherthan C group （224.03±9.90） U/ml （P〈0.05）. Conclusion Articular injection of sinomenine can decreased the levels ofNO and MDA and increase the activity of SOD in the serum. So it can protect the articular cartilage and play a preventiveeffect of genicular osteoarthritis.