检测IFN-γ和IL-4在急性胰腺炎中的应用

目的 探讨检测细胞因子干扰素（interferon,IFN）γ和白细胞介素（Interleukin IL）4的表达在急性胰腺炎（AP）的病情严重程度及疗效评估中的价值.方法 2009年11月～2013年6月,采用酶联免疫吸附试验（ELISA）分别测定32例轻型胰腺炎（MAP组）与18例重症胰腺炎（SAP组）患者住院第1,4,7天和15例正常健康人（正常对照组）的IFN γ和IL 4表达水平.结果 MAP组中住院第1,4,7天IFN γ的检测结果（ng/L）分别为31.47±4.35,36.92±4.12,49.09±4.97,IL 4的表达水平（ng/L）为54.72±5.52,45.26±4.89,39.79±3.85.SAP组中住院第1,4,7天IFN γ表达水平（ng/L）为23.97±5.39,26.29±3.47,33.64±4.47;IL-4的表达水平（ng/L）为70.45±5.05,65.99±4.58,56.23±4.23;正常对照组IFN γ和IL 4的检测结果（ng/L）分别为49.26±20.67,35.15±16.32.与正常对照组相比,MAP组（除外第7天）和SAP组患者外周血IFN-γ表达降低,IL 4表达升高,差异有统计学意义（t=0.753～4.288,P＜0.05）;相同时间点,SAP组各检测值与MAP组比较差异有统计学意义（t=3.248～11.416,P＜0.05）.结论 AP的病情进展与患者血清IFN-γ水平降低或者IL 4水平增高有关,检测细胞因子IFN-γ和IL 4有助于判断AP的病情严重程度和治疗效果.

Significance of Combined Detection of Helper T Lymphocyte 1 and 2 Type Cytokines in Acute Pancreatitis

Objective To explore the value of detection of Interferon （IFN）-γ and Interleukin（IL）-4 expression in acute pancreatitis（AP） severity and efficacy evaluation.Methods From May 2009 to June 2013,using enzyme-linked immunosorbent assay （ELISA）,the expression levels of Th1-type cytokines interferon-γ（IFN-γ） and Th2 cytokines interleukin （Interleukin IL） 4 of 32 cases of mild acute pancreatitis （MAP group）and 18 cases of severe acute pancreatitis （SAP group）at 1,4,7 d hospitalized as well as 15 cases of normal healthy people （control group） were determined.Results In MAP group hospitalization1,4,7d,thetest results ofIFN-γ （ng/L） were 31.47±4.35,36.92±4.12,49.09±4.97,and those results of IL-4 （ng/L） were 54.72±5.52,45.26±4.89,39.79±3.85.In SAP group hospitalization 1,4d and 7d,the test results of IFN-γ （ng/L） were 23.97±5.39,26.29±3.47,33.64±4.47,and those results of IL 4 （ng/L） were 70.45±5.05,65.99±4.58,56.23±4.23.In the normal control group,the test results of IFN-γ and IL 4 （ng/L） were 49.26±20.67 and 35.15±16.32 respectively.Compared with normal control group,the expression of Th1 type cytokines IFN-γ was decreased and that of Th2 type cytokine IL 4 was increased in MAP group （except for the 7th day） and SAP group patients,and the difference was statistically significant （t=0.753～4.288,P＜0.05）;the test results of each same time were statistically significant differences between SAP group and MAPgroup （t=3.248～11.416,P＜0.05）.Conclusion The progression of acute pancreatitis was related to the decreasion of IFN γ or increasion of IL-4 and combined detection of IFN-γ and IL 4 is beneficial to judge the extent of serious condition and treatment of acute pancreatitis.