二甲氨基乙醇和复方氨基酸溶液对衰老大鼠皮肤MMP-1和TIMP-1mRNA表达的影响

目的依据Mesotherapy的治疗理念，观察真皮内注射二甲氨基乙醇（DMAE）和复方氨基酸溶液（AA）对D-半乳糖诱导的化学性衰老大鼠皮肤MMP-1和TIMP-1mRNA表达的影响。方法80只wistar大鼠随机分人正常对照组、衰老对照组、衰老治疗组。衰老治疗组从注射D．半乳糖后第18天开始，在大鼠两侧臀部皮肤分别皮内注射0．2％DMAE＋AA、0．1％DMAE＋AA、0．2％DMAE、0．1％DMAE、AA、生理盐水（NS），每周1次，连续4周。观察各组皮肤组织学、真皮厚度、羟脯氨酸（Hyp）、MMP-1、TIMP-1mRNA和成纤维细胞核增殖抗体（PCNA）表达水平的变化。结果真皮内注射0．1％DMAE＋AA、0．2％DMAE＋AA，可使衰老大鼠真皮厚度、Hyp含量和MMP-1mRNA表达水平显著增加，而对TIMP-1mRNA和PCNA的表达没有影响。结论在衰老大鼠真皮内注射0．2％DMAE＋AA、0．1％DMAE＋AA，可促进老化、变性胶原蛋白分解，重构衰老皮肤组织结构，使皮肤中胶原蛋白含量显著增多，真皮厚度增加．具有一定延缓皮肤衰老的作用。

Effect of dimethylaminoethano and compound amino acid on MMP-1 and TIMP-1 mRNA expression of rat aging skin

Objective To study the effect of dimethylaminoethano and compound amino acid on MMP-1 and TIMP-1 mRNA expression of rat aging skin induced by D-galactose. Methods Eighty wistar rats were ran- domly divided into the normal control group, the aging control group and the experimental groups. At 18 days af- ter D-gal induction, the experimental groups were treated with intradermal microinjeetion of 0.2% DMAE ＋ AA, 0. 1%DMAE ＋ AA,0. 2% DMAE,0.1% DMAE,AA and normal saline （NS） on bilateral buttocks respectively, once a week for 4 weeks. At 42 days after treatment, the histological changes, the dermal thickness, hydroxypro- line （Hyp） and mRNA expression of MMP-1, TIMP-1 and PCNA in every group were detected. Results Meso- therapy with intradermal co-injection of 0.1% DMAE ＋ AA, or 0.2% DMAE ＋ AA significantly increased the der- mal thickness, Hydroxyproline content and MMP-1 mRNA expression levels, but did not affect the expression of TIMP-1 mRNA and PCNA in aging rats. Conclusion Local co-injection of DMAE and compound AA directly into target tissue under mesotherapy has marked anti-aging effects on D-galactose induced skin aging model of rat by degradation of aging collagen fiber and reconstruction of skin structure.