白细胞介素-32在大鼠肾脏缺血再灌注损伤中的作用及其机制 |
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引用本文: | 曾莎青,;张达雄,;王育斌.白细胞介素-32在大鼠肾脏缺血再灌注损伤中的作用及其机制[J].数理医药学杂志,2014(3):271-274. |
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作者姓名: | 曾莎青 ;张达雄 ;王育斌 |
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作者单位: | [1]武汉亚洲心脏病医院灌注科,武汉430022; [2]武汉科技大学医学院,武汉430022; |
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摘 要: | 目的:探讨白细胞介素-32(Interleukin,IL-32)在大鼠肾缺血再灌注(ischemia-reperfusion injury,IRI)损伤中的作用及其机制。方法:80只雄性SD大鼠随机分为4组:假手术组(S组,n=20)、缺血再灌注组(I/R组,n=20)、缺血前IgG抗体预处理组(IgG+I/R组,n=20)和缺血前IL-32抗体预处理组(Anti-IL-32+I/R组,n=20)。采用夹闭双侧肾蒂30min后恢复血供的方法制备肾脏缺血再灌注损伤模型,再灌注3h、6h、12h、24h分别处死大鼠收集血样和肾脏样本,自动生化分析仪测定血清中肌酐(Cr)、尿素氮(BUN)浓度,酶联免疫吸附试验(ELISA)检测大鼠血清IL-32、TNF-α、IL-1β的表达,化学比色法检测肾组织髓过氧化物酶(MPO)的活性。结果:与S组比较:1、血清Cr、BUN浓度,I/R、IgG+I/R组均显著升高(P0.05),Anti-IL-32+I/R组无显著性差异(P0.05);2、血清IL-32、TNF-α、IL-1β水平,I/R、IgG+I/R组均显著升高(P0.05),Anti-IL-32+I/R组无显著性差异(P0.05);3、肾组织MPO活性,I/R、IgG+I/R组显著增强(P0.05),Anti-IL-32+I/R组无显著性差异(P0.05)。结论:IL-32在肾脏缺血再灌注损伤后的大鼠血清中高表达,阻断IL-32能减少炎症因子释放、抑制中性粒细胞聚集,减轻肾功能损害。
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关 键 词: | 肾缺血再灌注损伤 白细胞介素-32 炎症因子 髓过氧化物酶 |
Effect of IL-32 on Renal Ischemia-reperfusion Injury and its Mechanism in Rats |
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Institution: | Zeng Shaqing, et al (Department of Perfusion, Wuhan ASIA Heart Hospital, Wuhan 430022) |
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Abstract: | Objective:To investigate the effect of IL-32on renal ischemia-reperfusion injury and its mechanism in rats.Methods:Eighty male SD rats were randomly divided into 4groups:Sham group(S group,n=20),renal IRI group(I/R group,n=20),IgG preconditioning before ischemia group(IgG+I/R group,n=20)and Anti-IL-32preconditioning before ischemia group(Anti-IL-32+I/R group,n=20).Both renal pedicales of rats were clamped for 30minutes to make the animal models of renal ischemia-reperfusion injury,then removed the clamps.3h,6h,12h,24hafter renal reperfusion,the plasma and kidneys were collected for detecting the serum creatinine,BUN level by auto biochemical analysis,IL-32,TNF-αand IL-1βexpression in serum by ELISA assay,activity of myeloperoxidase in kidney tissues by chemical chromatometry.Results:The levels of serum Cr and BUN in I/R group and IgG+I/R group were significantly higher than S group(P〈0.05),but there is no significant difference between Anti-IL-32+I/R group and S group(P〈0.05).Similarly,The levels of IL-32,TNF-αand IL-1βin I/R and IgG+I/R group were significantly higher than S group(P〈0.05),but there is no significant difference between Anti-IL-32+I/R group and S group(P〈0.05).The activity of MPO reduced significantly(P〈0.05).Conclusions:IL-32is highly expressed in serum and kidney on renal ischemia-reperfusion injury.Blockages of IL-32could decrease the level of inflammatory,inhibit the movement of neutrophils,and reduce the renal histopathologic damage. |
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Keywords: | renal ischemia-reperfusion injury IL-32 inflammatory factors myeloperoxidase(MPO) |
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